Eight novel single chain antibody fragments recognising VP2 of foot-and-mouth disease virus serotypes A, O, and SAT 2.
Virology
; 533: 145-154, 2019 07.
Article
en En
| MEDLINE
| ID: mdl-31170612
ABSTRACT
Foot-and-mouth disease virus (FMDV) exhibits a high degree of antigenic diversity among its serotypes, requiring several anti-FMDV antibodies for its laboratory diagnosis, which complicated the used techniques. To conquer this cumbersome, we developed a new panel of different single-chain fragment variable (scFv) for serotype-independent detection of FMDV. The recombinant VP2 capsid protein, as a relatively conserved protein among FMDV serotypes, was expressed in E. Coli, and injected in mice. Spleen's RNA was extracted for isolating the coding sequences of IgG variable domains that were assembled into repertoires of scFv. Phage library displaying scFv was constructed with â¼1.9â¯×â¯108 plaque forming units. Characterization of the library showed eight of unique scFvs, which were expressed as bacterial periplasmic proteins with apparent molecular weight of â¼27â¯kDa. Our data revealed the broad-spectrum binding affinity of the eight scFvs as both coating and tracing antibodies to FMDV serotypes A, O, and SAT 2.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Virus de la Fiebre Aftosa
/
Proteínas de la Cápside
/
Anticuerpos de Cadena Única
/
Fiebre Aftosa
Tipo de estudio:
Diagnostic_studies
Límite:
Animals
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Female
/
Humans
Idioma:
En
Revista:
Virology
Año:
2019
Tipo del documento:
Article