Diagnostic value of LncRNA-MEG3 as a serum biomarker in patients with hepatitis B complicated with liver fibrosis.
Eur Rev Med Pharmacol Sci
; 23(10): 4360-4367, 2019 May.
Article
en En
| MEDLINE
| ID: mdl-31173310
ABSTRACT
OBJECTIVE:
The aim of this work was to explore whether lncRNA-MEG3 could serve as a serum biomarker for diagnosing chronic hepatitis B (CHB) and improve the early diagnostic and treatment efficacies. PATIENTS ANDMETHODS:
Serum level of lncRNA-MEG3 in CHB patients and healthy controls was detected by quantitative Real Time-Polymerase Chain Reaction (qRT-PCR). Subsequently, CHB patients were divided into HBeAg-positive group and HBeAg-negative group based on the infection condition of hepatitis B virus. Correlation analyses were conducted to investigate the relationship between lncRNA-MEG3 level and HBV. Correlation between serum level of lncRNA-MEG3 and liver fibrosis was also analyzed. Survival analyses were performed to elucidate whether lncRNA-MEG3 could be served as a serum biomarker for diagnosing CHB combined with liver fibrosis. Expression levels of lncRNA-MEG3, α-SMA, and COL1A1 in mouse hepatic stellate cells (HSCs) were detected.RESULTS:
Serum level of lncRNA-MEG3 was lower in CHB patients compared with that of healthy controls, which was negatively correlated to liver fibrotic degree. Survival analyses showed that serum level of lncRNA-MEG3 exerts significant diagnostic value on the liver fibrotic degree in CHB patients. ROC (receiver operating curve) results showed the AUC was 0.9395, the sensitivity was 100%, and the specificity was 78.13% in comparing the serum level of lncRNA-MEG3 between CHB patients with liver fibrosis and healthy controls. Further analyses showed that serum level of lncRNA-MEG3 was negatively correlated to levels of α-SMA and COL1A1. However, no significant correlations were found among the serum level of lncRNA-MEG3, HBV, hepatic inflammation and liver function. In vitro experiments showed that lncRNA-MEG3 expression was gradually decreased, whereas expression levels of α-SMA and COL1A1 in HSCs were gradually increased in a time-dependent manner.CONCLUSIONS:
Serum level of lncRNA-MEG3 is lowly expressed in CHB patients, which is negatively correlated to the liver fibrotic degree. LncRNA-MEG3 may serve as a diagnostic biomarker for CHB.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Biomarcadores
/
ARN Largo no Codificante
/
Hepatitis B
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Cirrosis Hepática
Tipo de estudio:
Diagnostic_studies
/
Prognostic_studies
Límite:
Adult
/
Animals
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Female
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Humans
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Male
/
Middle aged
Idioma:
En
Revista:
Eur Rev Med Pharmacol Sci
Asunto de la revista:
FARMACOLOGIA
/
TOXICOLOGIA
Año:
2019
Tipo del documento:
Article
País de afiliación:
China