[Oxidative Damage of Bone Marrow Stromal Cells Caused by Chemotherapy Drugs].
Zhongguo Shi Yan Xue Ye Xue Za Zhi
; 27(3): 970-975, 2019 Jun.
Article
en Zh
| MEDLINE
| ID: mdl-31204963
ABSTRACT
OBJECTIVE:
To explore the oxidative damage of OP9 cells induced by daunorubicin (DNR) treatment.METHODS:
The TMRM probe was used to detect mitochondrial membrane potential by flow cytometry; the reactive oxygen species (ROS) was determined by flow cytometry DCFDA probe; the real-time PCR was used to detect the molecular expression of antioxidant enzymeï¼glutathione peroxidase (GPX) in OP9 cells; the expression of γ-H2AX was determined by flow cytometry.RESULTS:
Compared with normal OP9 cells, the positive rate of TMRM in DNR-treated OP9 cells decreased by 56.7% (Pï¼0.05); the positive rate of DCFDA in DNR-treated OP9 cells increased by 3.52 times (Pï¼0.01). Compared with normal OP9 cells, DNR-treated OP9 cells showed a decrease in the expression of GPX4 by 44.22% (Pï¼0.001); the expression of GPX7 decreased by 65.7% (Pï¼0.001); the expression of GPX8 decreased by 24.7% (Pï¼0.001); the positive rate of γ-H2AX in DNR-treated OP9 cells increased (Pï¼0.05).CONCLUSION:
After DNR treatment, mitochondrial membrane potential of OP9 cells decreases; the level of reactive oxygen species increases; the expression of glutathione peroxidase (GPX) molecules decreases significantly; genomic instability increases obviously; the oxidative damage of cells increased.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Células Madre Mesenquimatosas
Idioma:
Zh
Revista:
Zhongguo Shi Yan Xue Ye Xue Za Zhi
Asunto de la revista:
HEMATOLOGIA
Año:
2019
Tipo del documento:
Article