Plasmonic Selection of ssDNA Aptamers against Fibroblast Growth Factor Receptor.
ACS Comb Sci
; 21(8): 578-587, 2019 08 12.
Article
en En
| MEDLINE
| ID: mdl-31265241
ABSTRACT
In this work, we describe the selection of ssDNA aptamers targeting fibroblast growth factor receptor binding protein 3 K650E, which has roles in cell division, growth, and differentiation through the kinase cascade. The selection process was based on the label-free, real-time monitoring of binding interactions by surface plasmon resonance, allowing for convenient manipulation of the selection rounds. Next generation sequencing data provided four major motif families from which nine individual sequences were selected based on their abundance levels. Electrophoretic mobility shift assays revealed binding of the selected aptamers to the target protein without significant interference from fibroblast growth factor receptor binding protein 2, indicating the selectivity of the aptamers. The dissociation constant at equilibrium for the best aptamer candidate, SU-3, was found to be (28.2 ± 19.6) × 10-9 M (n = 5) using a single-cycle kinetic analysis method. Advantages of the experimental setup and potential applications of the selected aptamers are discussed.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
ADN de Cadena Simple
/
Receptores de Factores de Crecimiento de Fibroblastos
/
Aptámeros de Nucleótidos
Límite:
Humans
Idioma:
En
Revista:
ACS Comb Sci
Año:
2019
Tipo del documento:
Article
País de afiliación:
Turquía