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The Zymoseptoria tritici ORFeome: A Functional Genomics Community Resource.
Chaudhari, Yogesh; Cairns, Timothy C; Sidhu, Yaadwinder; Attah, Victoria; Thomas, Graham; Csukai, Michael; Talbot, Nicholas J; Studholme, David J; Haynes, Ken.
Afiliación
  • Chaudhari Y; Biosciences, University of Exeter, Exeter EX4 4QD, U.K.
  • Cairns TC; Biosciences, University of Exeter, Exeter EX4 4QD, U.K.
  • Sidhu Y; Biosciences, University of Exeter, Exeter EX4 4QD, U.K.
  • Attah V; Biosciences, University of Exeter, Exeter EX4 4QD, U.K.
  • Thomas G; Biosciences, University of Exeter, Exeter EX4 4QD, U.K.
  • Csukai M; Syngenta, Jealott's Hill International Research Centre, Bracknell, RG42 6EY, U.K.
  • Talbot NJ; The Sainsbury Laboratory, University of East Anglia, Norwich Research Park, NR47UH, U.K.
  • Studholme DJ; Biosciences, University of Exeter, Exeter EX4 4QD, U.K.
  • Haynes K; Biosciences, University of Exeter, Exeter EX4 4QD, U.K.
Mol Plant Microbe Interact ; 32(12): 1564-1570, 2019 Dec.
Article en En | MEDLINE | ID: mdl-31272284
ABSTRACT
Libraries of protein-encoding sequences can be generated by identification of open reading frames (ORFs) from a genome of choice that are then assembled into collections of plasmids termed ORFeome libraries. These represent powerful resources to facilitate functional genomic characterization of genes and their encoded products. Here, we report the generation of an ORFeome for Zymoseptoria tritici, which causes the most serious disease of wheat in temperate regions of the world. We screened the genome of strain IP0323 for high confidence gene models, identifying 4,075 candidates from 10,933 predicted genes. These were amplified from genomic DNA, were cloned into the Gateway entry vector pDONR207, and were sequenced, providing a total of 3,022 quality-controlled plasmids. The ORFeome includes genes predicted to encode effectors (n = 410) and secondary metabolite biosynthetic proteins (n = 171) in addition to genes residing at dispensable chromosomes (n = 122) or those that are preferentially expressed during plant infection (n = 527). The ORFeome plasmid library is compatible with our previously developed suite of Gateway destination vectors, which have various combinations of promoters, selection markers, and epitope tags. The Z. tritici ORFeome constitutes a powerful resource for functional genomics and offers unparalleled opportunities to understand the biology of Z. tritici.[Formula see text] Copyright © 2019 The Author(s). This is an open access article distributed under the CC BY 4.0 International license.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ascomicetos / Biblioteca Genómica / Sistemas de Lectura Abierta / Genoma Fúngico / Genómica Tipo de estudio: Prognostic_studies Idioma: En Revista: Mol Plant Microbe Interact Asunto de la revista: BIOLOGIA MOLECULAR / BOTANICA / MICROBIOLOGIA Año: 2019 Tipo del documento: Article País de afiliación: Reino Unido

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ascomicetos / Biblioteca Genómica / Sistemas de Lectura Abierta / Genoma Fúngico / Genómica Tipo de estudio: Prognostic_studies Idioma: En Revista: Mol Plant Microbe Interact Asunto de la revista: BIOLOGIA MOLECULAR / BOTANICA / MICROBIOLOGIA Año: 2019 Tipo del documento: Article País de afiliación: Reino Unido