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Exploring the analytical power of the QTOF MS platform to assess monoclonal antibodies quality attributes.
Gomes, Ricardo A; Almeida, Conceição; Correia, Catarina; Guerreiro, Ana; Simplício, Ana Luísa; Abreu, Isabel A; Alves, Patrícia Gomes.
Afiliación
  • Gomes RA; UniMS - Mass Spectrometry Unit, ITQB/IBET, Oeiras, Portugal.
  • Almeida C; UniMS - Mass Spectrometry Unit, ITQB/IBET, Oeiras, Portugal.
  • Correia C; UniMS - Mass Spectrometry Unit, ITQB/IBET, Oeiras, Portugal.
  • Guerreiro A; UniMS - Mass Spectrometry Unit, ITQB/IBET, Oeiras, Portugal.
  • Simplício AL; UniMS - Mass Spectrometry Unit, ITQB/IBET, Oeiras, Portugal.
  • Abreu IA; UniMS - Mass Spectrometry Unit, ITQB/IBET, Oeiras, Portugal.
  • Alves PG; UniMS - Mass Spectrometry Unit, ITQB/IBET, Oeiras, Portugal.
PLoS One ; 14(7): e0219156, 2019.
Article en En | MEDLINE | ID: mdl-31291294
The biopharmaceutical industry is growing at a fast pace, making nowadays 20% of the pharma market. Within this market, therapeutic monoclonal antibodies (mAbs) are the dominant product class. With the patent expirations, biosimilars and, perhaps more relevant, biobetters, are in fast development. Thus, a comprehensive characterization at the molecular level of antibodies heterogeneity such as glycoforms, post-translational modifications (PTMs) and sequence variations is of utmost importance. Mass spectrometry (MS)-based approaches are undoubtedly the most powerful analytical strategies to monitor and define an array of critical quality attributes on mAbs. In this work, we demonstrate the analytical power of the Q-TOF MS platform for comprehensive and detailed analysis at molecular levels of an in-house produced mAb. This methodology involves minimal sample preparation procedures and provides an extensive collection of valuable data in a short period of time.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Espectrometría de Masas / Anticuerpos Monoclonales Límite: Humans Idioma: En Revista: PLoS One Asunto de la revista: CIENCIA / MEDICINA Año: 2019 Tipo del documento: Article País de afiliación: Portugal Pais de publicación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Espectrometría de Masas / Anticuerpos Monoclonales Límite: Humans Idioma: En Revista: PLoS One Asunto de la revista: CIENCIA / MEDICINA Año: 2019 Tipo del documento: Article País de afiliación: Portugal Pais de publicación: Estados Unidos