Simultaneous extraction and separation of oil, proteins, and glucosinolates from Moringa oleifera seeds.
Food Chem
; 300: 125162, 2019 Dec 01.
Article
en En
| MEDLINE
| ID: mdl-31325745
ABSTRACT
Moringa oleifera is a worldwide cultivated edible and medicinal plant. Its seeds are rich in oil, proteins, and glucosinolates. A practical method was developed to simultaneously extract and separate the three groups of substances from M. oleifera seeds. Smashed seed material was loaded into columns with petroleum ether ethanol 82 (PE-ethanol) and eluted sequentially with 4.8-fold PE-ethanol to extract oil, and 10.8-fold water to extract proteins and glucosinolates. More than 95% of oil, proteins, and glucosinolates were extracted. The extracts were separated automatically into ether (oil) phase and ethanol aqueous phase. The latter was further separated into proteins and glucosinolates by 70% ethanol precipitation. The main glucosinolate was identified by LC-MS as GLC (4-α-rhamnopyranosyloxy-benzyl glucosinolate). After purification, 22.3â¯g refined oil, 33.0â¯g proteins, and 5.5â¯g purified GLC from 100â¯g M. oleifera seeds were obtained. This study provides a simple and high-efficient method to utilize M. oleifera seeds.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Proteínas de Plantas
/
Espectrometría de Masas
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Aceites de Plantas
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Cromatografía Liquida
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Moringa oleifera
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Glucosinolatos
Idioma:
En
Revista:
Food Chem
Año:
2019
Tipo del documento:
Article
País de afiliación:
China