Effects of 3300 del A-1061 Ter BRCA1 frameshift mutation and calcium propionate on oxidative stress and breast carcinogenesis.

BRCA1 gene mutation increases risk of hereditary breast/ovarian carcinogenesis. Calcium propionate is the food preservative. Superoxide dismutase enzyme protects oxidative stress in human. This report studied about the effects of 3300 del A-1061 Ter BRCA1 mutation at exon 11 and calcium propionate toxicity on parameters of oxidative stress induction and cause of breast cancer. The effects of 3300 del A-1061 Ter BRCA1 frameshift mutation on oxidative stress protection were studied by MTT dye reduction assay and superoxide dismutase activity assay. Calcium propionate and 3300 del A-1061 Ter BRCA1 frameshift mutation effects on superoxide dismutase activity were studied by superoxide dismutase activity assay on breast cancer cell line. The results showed that this mutation caused oxidative stress through superoxide dismutase activity inhibition (P < 0.05) and calcium propionate effected on superoxide dismutase activity in breast cancer cells (P < 0.05). 3300 del A-1061 Ter BRCA1 mutation and the chemical toxicity effect on oxidative stress and may lead to breast carcinogenesis.