Preparation of a crude chitosanase from blue crab viscera as well as its application in the production of biologically active chito-oligosaccharides from shrimp shells chitosan.
Int J Biol Macromol
; 139: 558-569, 2019 Oct 15.
Article
en En
| MEDLINE
| ID: mdl-31340177
ABSTRACT
In this study, a digestive chitosanase from blue crab (Portunus segnis) viscera was extracted, characterized and applied. The crude chitosanase showed optimum activity at pHâ¯4.0 and 60⯰C and retained >80% of its activity over a pH range from 3.0 to 10.0. Subsequently, the crude chitosanase was applied to produce bioactive varying molecular weight (Mw) and acetylation degree chitosan-depolymerization products (CDP) with specially sequences composition determined by MALDI-TOF MS owing to an endo-cleavage mode. This hydrolysis process allowed to the preparation, after 24â¯h of incubation at 40⯰C, of a low Mw water soluble CDP (H 24h, <4.4â¯kDa) with DP up to 6 and a high Mw CDP (C 24h, 142.19â¯kDa). Following their physicochemical characterization, the functional properties, antioxidant and antimicrobial activities of CDP were investigated. Interestingly, as compared to the native chitosan, CDP, especially low Mw derivatives (H 24h) exhibited potent antioxidant activities, while high Mw derivatives, especially C 24h, markedly inhibited the growth of all tested bacteria and fungi. These results may provide novel insights into the efficiency of chitosan depolymerisation using the Portunus segnis digestive crude chitosanase as a simple, inexpensive and easily method to produce bioactive chitosan-derivatives and that this bioactivity depends highly on their attractive characteristics.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Oligosacáridos
/
Vísceras
/
Braquiuros
/
Quitosano
/
Exoesqueleto
/
Glicósido Hidrolasas
Límite:
Animals
Idioma:
En
Revista:
Int J Biol Macromol
Año:
2019
Tipo del documento:
Article
País de afiliación:
Túnez