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Creation of CRISPR-based germline-genome-engineered mice without ex vivo handling of zygotes by i-GONAD.
Gurumurthy, Channabasavaiah B; Sato, Masahiro; Nakamura, Ayaka; Inui, Masafumi; Kawano, Natsuko; Islam, Md Atiqul; Ogiwara, Sanae; Takabayashi, Shuji; Matsuyama, Makoto; Nakagawa, Shinichi; Miura, Hiromi; Ohtsuka, Masato.
Afiliación
  • Gurumurthy CB; Mouse Genome Engineering Core Facility, Vice Chancellor for Research Office, University of Nebraska Medical Center, Omaha, NE, USA. cgurumurthy@unmc.edu.
  • Sato M; Department of Pharmacology and Experimental Neuroscience, College of Medicine, University of Nebraska Medical Center, Omaha, NE, USA. cgurumurthy@unmc.edu.
  • Nakamura A; Section of Gene Expression Regulation, Frontier Science Research Center, Kagoshima University, Kagoshima, Japan.
  • Inui M; Support Center for Medical Research and Education, Tokai University, Kanagawa, Japan.
  • Kawano N; Laboratory of Animal Regeneration Systemology, Department of Life Science, School of Agriculture, Meiji University, Kanagawa, Japan.
  • Islam MA; Meiji University International Institute for Bio-Resource Research, Kanagawa, Japan.
  • Ogiwara S; Laboratory of Regulatory Biology, Department of Life Science, School of Agriculture, Meiji University, Kanagawa, Japan.
  • Takabayashi S; Division of Basic Medical Science and Molecular Medicine, School of Medicine, Tokai University, Kanagawa, Japan.
  • Matsuyama M; Laboratory of Laboratory Animal Science and Medicine, Graduate School of Veterinary Medicine, Hokkaido University, Hokkaido, Japan.
  • Nakagawa S; Support Center for Medical Research and Education, Tokai University, Kanagawa, Japan.
  • Miura H; Laboratory for Animal Resources Development, Hamamatsu University School Of Medicine, Hamamatsu, Shizuoka, Japan.
  • Ohtsuka M; Division of Molecular Genetics, Shigei Medical Research Institute, Minami-ku, Okayama, Japan.
Nat Protoc ; 14(8): 2452-2482, 2019 08.
Article en En | MEDLINE | ID: mdl-31341289
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Electroporación / Sistemas CRISPR-Cas / Edición Génica Límite: Animals / Pregnancy Idioma: En Revista: Nat Protoc Año: 2019 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Electroporación / Sistemas CRISPR-Cas / Edición Génica Límite: Animals / Pregnancy Idioma: En Revista: Nat Protoc Año: 2019 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido