Simultaneous quantification of lidocaine and prilocaine in human plasma by LC-MS/MS and its application in a human pharmacokinetic study.
Pract Lab Med
; 17: e00129, 2019 Nov.
Article
en En
| MEDLINE
| ID: mdl-31414038
ABSTRACT
OBJECTIVE:
The aim of the work was to develop and validate a simple, sensitive and selective Liquid chromatography with Mass spectroscopic method for simultaneous quantification of lidocaine and prilocaine in human plasma. DESIGN ANDMETHODS:
Analytes and the internal standards from human plasma were extracted by using solid- phase extraction technique using Waters Oasis® HLB 1 âcc (30 âmg) cartridges. The reconstituted samples were chromatographed on Phenomenex Kinetex EVO 4.6*100 âmm 2.6 µ 100A column by using a mixture of acetonitrile and 5 âmM ammonium acetate buffer (8020, v/v) as the mobile phase at a flow rate of 0.6 âmL/min.RESULTS:
The method was validated over the concentration range of 0.10-201.80â¯ng/mL for lidocaine and 0.10-201.66â¯ng/mL for prilocaine. The calibration curve obtained was linear.CONCLUSION:
Method validation was performed as per FDA guidelines and the results met the acceptance criteria. A run time of 3.0â¯min for each sample, make it possible to analyze more than 350 human plasma samples per day. The proposed method was found applicable for pharmacokinetic studies.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Idioma:
En
Revista:
Pract Lab Med
Año:
2019
Tipo del documento:
Article
País de afiliación:
India