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Online clean-up setup for the determination of non-fluorescent acidic pharmaceutical drugs in complex biological samples.
Muhammad, Nadeem; Guo, Dandan; Zhang, Yun; Intisar, Azeem; Subhani, Qamar; Qadir, Muhammad Abdul; Cui, Hairong.
Afiliación
  • Muhammad N; Department of Environmental Engineering, Wuchang University of Technology, Wuhan, China; Department of Chemistry, Xixi Campus, Zhejiang University, Hangzhou 310028, China. Electronic address: nadeem@zju.edu.cn.
  • Guo D; Department of Chemistry, Xixi Campus, Zhejiang University, Hangzhou 310028, China; Ningbo University, Institution of drug discovery technology, Ningbo 315211, Zhejiang, Peoples R. China. Electronic address: 327061889@qq.com.
  • Zhang Y; Department of Chemistry, Xixi Campus, Zhejiang University, Hangzhou 310028, China.
  • Intisar A; Institute of Chemistry, University of the Punjab, Lahore, Pakistan.
  • Subhani Q; Department of Chemistry, Xixi Campus, Zhejiang University, Hangzhou 310028, China; Higher Education Department, Lahore, Punjab, Pakistan.
  • Qadir MA; Institute of Chemistry, University of the Punjab, Lahore, Pakistan.
  • Cui H; Department of Environmental Engineering, Wuchang University of Technology, Wuhan, China.
J Chromatogr B Analyt Technol Biomed Life Sci ; 1126-1127: 121708, 2019 Sep 15.
Article en En | MEDLINE | ID: mdl-31437773
ABSTRACT
Analysis of acidic pharmaceuticals in complex biological samples is a challenging and formidable task due to the existence of interfering constituents within the sample matrices. Therefore, in order to avoid analytical column clogging and suppression/enhancement of signals of the analyte of interest, herein a simple, cost-effective and quick online ion chromatography based clean-up setup was introduced. This system was further coupled with a cost-effective homemade photochemically induced fluorimetric (PIF) setup for direct online conversion of non-fluorescent acidic pharmaceutical drugs into their respective fluorescent species. This advantageous system was favorably applied for the determination of four non-fluorescent acidic compounds in two complex biological samples (human serum and oral fluid) with minimum labor and organic solvent consumption. At optimized conditions, the developed method has shown good sensitivity, selectivity, satisfactory recoveries (88.68-102.14%) and low limits of detection (0.35-8.10 µg/L) with minimum or zero matrix effect.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Preparaciones Farmacéuticas / Cromatografía por Intercambio Iónico / Fluorometría Tipo de estudio: Prognostic_studies Límite: Humans Idioma: En Revista: J Chromatogr B Analyt Technol Biomed Life Sci Asunto de la revista: ENGENHARIA BIOMEDICA Año: 2019 Tipo del documento: Article
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Preparaciones Farmacéuticas / Cromatografía por Intercambio Iónico / Fluorometría Tipo de estudio: Prognostic_studies Límite: Humans Idioma: En Revista: J Chromatogr B Analyt Technol Biomed Life Sci Asunto de la revista: ENGENHARIA BIOMEDICA Año: 2019 Tipo del documento: Article