High-performance electrochemical enzyme sensor for organophosphate pesticide detection using modified metal-organic framework sensing platforms.
Bioelectrochemistry
; 130: 107348, 2019 Dec.
Article
en En
| MEDLINE
| ID: mdl-31437810
ABSTRACT
A practical electrochemical biosensor with high sensitivity was developed for detecting organophosphorus (OP). Initially, Ce metal was introduced into an UiO-66-template to form Ce/UiO-66. Later, graphene oxide (GO), carbon black (CB) and multi-walled carbon nanotubes (MWCNTs) were separately added to Ce/UiO-66 to compare the effect of different carbon-based material types on the performance of the biosensor. Exclusively, Ce/UiO-66/MWCNTs with a Ce (7%) and MWCNT (30%) matrix was found to not only load more acetylcholinesterase (AChE) onto vacant sites but also increase electron transfer and decrease the number of diffusion pathways between the thiocholine and electrode surface. Moreover, the appropriate oxophilicity of Ce coupled with the high surface area and good conductivity of MWCNTs in the UiO-66 structure revealed a high affinity to acetylthiocholine chloride (ATCl) and possible catalysis of the hydrolysis of ATCl with a Michaelis-Menten constant of 0.258â¯mM. This biosensor, under optimal conditions, demonstrated a rapid and sensitive detection of paraoxon over a wide linear range of 0.01-150â¯nM, with a low detection limit of 0.004â¯nM. As a result, the AChE/Ce/UiO-66/MWCNTs/GCE biosensor can be employed in laboratory and field experiments to determine paraoxon levels.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Paraoxon
/
Plaguicidas
/
Técnicas Biosensibles
/
Cerio
/
Estructuras Metalorgánicas
Tipo de estudio:
Diagnostic_studies
Límite:
Animals
Idioma:
En
Revista:
Bioelectrochemistry
Asunto de la revista:
BIOQUIMICA
Año:
2019
Tipo del documento:
Article
País de afiliación:
Irán
Pais de publicación:
HOLANDA
/
HOLLAND
/
NETHERLANDS
/
NL
/
PAISES BAJOS
/
THE NETHERLANDS