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Epigenetic aspects of floral homeotic genes in relation to sexual dimorphism in the dioecious plant Mercurialis annua.
Khadka, Janardan; Yadav, Narendra Singh; Guy, Micha; Grafi, Gideon; Golan-Goldhirsh, Avi.
Afiliación
  • Khadka J; French Associates Institute for Agriculture and Biotechnology of Drylands, Jacob Blaustein Institutes for Desert Research, Ben-Gurion University of the Negev, Midreshet Ben Gurion, Israel.
  • Yadav NS; Shanghai Jiao Tong University, China.
  • Guy M; French Associates Institute for Agriculture and Biotechnology of Drylands, Jacob Blaustein Institutes for Desert Research, Ben-Gurion University of the Negev, Midreshet Ben Gurion, Israel.
J Exp Bot ; 70(21): 6245-6259, 2019 11 18.
Article en En | MEDLINE | ID: mdl-31504768
In plants, dioecy characterizes species that carry male and female flowers on separate plants and it occurs in about 6% of angiosperms; however, the molecular mechanisms that underlie dioecy are essentially unknown. The ability for sex-reversal by hormone application raises the hypothesis that the genes required for the expression of both sexes are potentially functional but are regulated by epigenetic means. In this study, proteomic analysis of nuclear proteins isolated from flower buds of females, males, and feminized males of the dioecious plant Mercurialis annua revealed differential expression of nuclear proteins that are implicated in chromatin structure and function, including floral homeotic proteins. Focusing on floral genes, we found that class B genes were mainly expressed in male flowers, while class D genes, as well as SUPERMAN-like genes, were mainly expressed in female flowers. Cytokinin-induced feminization of male plants was associated with down-regulation of male-specific genes concomitantly with up-regulation of female-specific genes. No correlation was found between the expression of class B and D genes and the changes in DNA methylation or chromatin conformation of these genes. Thus, we could not confirm DNA methylation or chromatin conformation of floral genes to be the major determinant regulating sexual dimorphisms. Instead, determination of sex in M. annua might be controlled upstream of floral genes by one or more sex-specific factors that affect hormonal homeostasis. A comprehensive model is proposed for sex-determination in M. annua.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Genes Homeobox / Caracteres Sexuales / Genes de Plantas / Euphorbiaceae / Epigénesis Genética Tipo de estudio: Prognostic_studies Idioma: En Revista: J Exp Bot Asunto de la revista: BOTANICA Año: 2019 Tipo del documento: Article País de afiliación: Israel Pais de publicación: Reino Unido

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Genes Homeobox / Caracteres Sexuales / Genes de Plantas / Euphorbiaceae / Epigénesis Genética Tipo de estudio: Prognostic_studies Idioma: En Revista: J Exp Bot Asunto de la revista: BOTANICA Año: 2019 Tipo del documento: Article País de afiliación: Israel Pais de publicación: Reino Unido