Chitosan as flocculant for concentrating Euglena gracilis cultures.
Ann Inst Pasteur Microbiol
; 139(6): 717-26, 1988.
Article
en En
| MEDLINE
| ID: mdl-3150942
ABSTRACT
The practical criteria for the usefulness of an algal separation process for laboratory routine being effectiveness and time consumption, we tested the feasibility of a flocculation procedure to harvest large volumes of Euglena gracilis in culture. This procedure turned out to be a technically viable system which avoided tedious centrifugation and preserved E. gracilis flagellar apparatus integrity. E. gracilis cultures were treated with chitosan, a by-product derived from chitin from the exoskeleton of crustaceans. Since this polymer carries a positive charge, it functions as a polycationic coagulating agent by adsorbing onto particles in suspension and by bridging together into agglomerates, or flocs. A 96-98% reduction of suspended cells in cultures with 200 mg/l of chitosan, at pH 7.5, was obtained.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Quitina
/
Euglena gracilis
Límite:
Animals
Idioma:
En
Revista:
Ann Inst Pasteur Microbiol
Asunto de la revista:
MICROBIOLOGIA
Año:
1988
Tipo del documento:
Article
País de afiliación:
Italia