Plasma amyloid beta level changes in aged mice with cognitive dysfunction following sevoflurane exposure.
Exp Gerontol
; 129: 110737, 2020 01.
Article
en En
| MEDLINE
| ID: mdl-31521721
ABSTRACT
INTRODUCTION:
Previous studies have stated that cognitive impairment induced by anesthetics was associated with amyloid beta (Aß). However, few researchers have investigated the transport of Aß inside and outside of the brain.AIM:
We attempted to probe the effects of sevoflurane on cognitive functions, the plasma Aß, and transporters of Aß in aged mice. The receptor for advanced glycation end-products (RAGE) is an Aß influx protein, and Low-density lipoprotein receptor-related protein-1 (LRP-1) is an Aß efflux protein.METHODS:
Aged mice were divided into the control group and the sevoflurane group. The mice were exposed to 100% oxygen or 2.5% sevoflurane for 2â¯h. The abilities of spatial learning and memory in mice were tested using the Morris water maze. Aß concentrations of plasma were measured with enzyme-linked immunosorbent assay kits. The RAGE and LRP-1 gene levels in the brain were assessed with quantitative polymerase chain reaction, and the protein levels were determined by western blot analysis. The locations of RAGE in the brain were confirmed via immunofluorescence.RESULTS:
In the sevoflurane group mice, the escape latency was increased on the 5th day of training, and the time spent in the target quadrant was decreased on the 7th day after anesthesia. Sevoflurane reduced the concentration of plasma Aß1-40. In addition, sevoflurane increased both gene and protein levels of RAGE in the brain, and increased RAGE proteins co-localized with the hippocampal vascular endothelial cells.CONCLUSION:
RAGE over-expression in the hippocampal vascular endothelial cells possibly resulted in the excessive transport of the plasma Aß1-40 into the brain after treatment with sevoflurane, which was associated with sevoflurane-induced cognitive dysfunction in aged mice.Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Plasma
/
Péptidos beta-Amiloides
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Anestésicos por Inhalación
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Disfunción Cognitiva
/
Sevoflurano
Límite:
Animals
Idioma:
En
Revista:
Exp Gerontol
Año:
2020
Tipo del documento:
Article
País de afiliación:
China