Evaluation of serum biomarkers and efficacy of MTX in women with ectopic pregnancy.
Mol Med Rep
; 20(3): 2902-2908, 2019 Sep.
Article
en En
| MEDLINE
| ID: mdl-31524242
ABSTRACT
Ectopic pregnancy occurs when a fertilized ovum attaches outside the uterus. As a complication in approximately 12% of all pregnancies, ectopic pregnancies may cause catastrophic hemorrhage as a result of invading maternal blood vessels. Therefore, early diagnosis and timely treatment are crucial for women with ectopic pregnancy. In this study, we aimed to identify and determine the efficacy of serum biomarkers for the prompt diagnosis of ectopic pregnancy. For this purpose, the serum concentrations of progesterone, ß human chorionic gonadotropin (ßhCG) and cancer antigen125 (CA125) were detected by solidphase, competitive binding chemiluminescent enzyme immunoassays. Flow cytometry was used to analyze the percentage of CD3+ T cells in women with ectopic pregnancy. Pathological analysis of tubal and villus tissues was performed by hematoxylin and eosin (H&E) staining. After receiving an injection of methotrexate (MTX), patients were examined by transvaginal ultrasound to detect the size of the echogenic mass. The results revealed that the serum levels of progesterone, ßHCG and CA125 were significantly decreased in women with ectopic pregnancy, whereas the percentage of CD3+ T cells was increased in women with ectopic pregnancy. Histopathological examination revealed blood clots with small tissue fragments of a tubaltype epithelium and incomplete pile structures. Five days after the MTX injection, an echogenic mass was found with a size of 1.7x1.2x1.6 cm that contained a gestational saclike structure and a yolk sac. On the whole, the findings of this study indicate the at the joint detection of progesterone, ßHCG, CA125 serum levels and the CD3+ T cell percentage could be applied as a reliable indicator for the early diagnosis of ectopic pregnancy. MTX administration was determined to be an efficacious approach for the treatment of ectopic pregnancy.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Embarazo Ectópico
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Biomarcadores
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Metotrexato
Tipo de estudio:
Diagnostic_studies
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Prognostic_studies
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Screening_studies
Límite:
Adult
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Female
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Humans
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Pregnancy
Idioma:
En
Revista:
Mol Med Rep
Año:
2019
Tipo del documento:
Article