Computational design of a molecularly imprinted polymer compatible with an aqueous environment for solid phase extraction of chenodeoxycholic acid.
J Chromatogr A
; 1609: 460490, 2020 Jan 04.
Article
en En
| MEDLINE
| ID: mdl-31542206
ABSTRACT
The main problem of poor water compatibility of molecularly imprinted polymers (MIPs) has been addressed in this study. A new facile and highly efficient approach was developed to obtain well-defined hydrophilic molecularly imprinted polymer microsphere with excellent specific recognition ability toward Chenodeoxycholic acid (CDCA) in crude bile. Particularly, it involved computational modeling to obtain a polymer network with high affinity for CDCA and addition of a hydrophilic crosslinker (polyethylene glycol (PEG) diacrylateâ¼200) to increase the hydrophilicity of the polymer surface. To our knowledge, this study first report splitting method in molecular imprinting technology. By using the splitting method, simulation time can be saved under the premise of ensuring accuracy. The adsorption experiments revealed that an optimized CDCA-MIP exhibited better selectivity toward CDCA with inhibition of the nonspecific adsorption. The CDCA-MIP possessed adsorption capacity of 49.86â¯mg g-1 for CDCA and the imprinting factor was 2.72. Solid-phase extraction (SPE) using the prepared CDCA-MIP as adsorbent was optimized regarding loading and elution conditions, and it was used to extract CDCA from crude bile, resulting in recoveries in the range 94.2-96.1%.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Polímeros
/
Ácido Quenodesoxicólico
/
Extracción en Fase Sólida
/
Impresión Molecular
Tipo de estudio:
Prognostic_studies
Idioma:
En
Revista:
J Chromatogr A
Año:
2020
Tipo del documento:
Article
País de afiliación:
China