Aberrant DNA Hypermethylation Silenced LncRNA Expression in Gastric Cancer.
Anticancer Res
; 39(10): 5381-5391, 2019 Oct.
Article
en En
| MEDLINE
| ID: mdl-31570433
ABSTRACT
BACKGROUND/AIM:
Long noncoding RNAs (lncRNAs) are noncoding transcripts that are >200 nucleotides in length. However, the biological functions and regulation mechanisms of lncRNAs in gastric carcinogenesis remain unknown. MATERIALS ANDMETHODS:
The expression levels of Linc00472 were analyzed by real-time PCR. The DNA methylation status was assessed using Combined Bisulfite Restriction Analysis (COBRA). The biological role of Linc00472 was assessed in AGS cells with Linc00472 overexpression.RESULTS:
Using the next-generation sequencing approach, we identified DNA methylation-associated lncRNAs in gastric cancer cells. Among them, the expression level of Linc00472 significantly decreased in gastric cancer tissues compared to adjacent normal tissues. Furthermore, we observed a more frequent hypermethylation of CpG islands upstream of Linc00472 in gastric cancer tissues. Ectopic Linc00472 expression could significantly inhibit gastric cancer cell growth and migration.CONCLUSION:
Epigenetically regulated Linc00472 expression plays a crucial role in modulating gastric cancer cell growth and motility.Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Neoplasias Gástricas
/
Metilación de ADN
/
ARN Largo no Codificante
Límite:
Humans
Idioma:
En
Revista:
Anticancer Res
Año:
2019
Tipo del documento:
Article