Your browser doesn't support javascript.
loading
Differential requirements for cyclase-associated protein (CAP) in actin-dependent processes of Toxoplasma gondii.
Hunt, Alex; Russell, Matthew Robert Geoffrey; Wagener, Jeanette; Kent, Robyn; Carmeille, Romain; Peddie, Christopher J; Collinson, Lucy; Heaslip, Aoife; Ward, Gary E; Treeck, Moritz.
Afiliación
  • Hunt A; Signalling in Apicomplexan Parasites Laboratory, The Francis Crick Institute, London, United Kingdom.
  • Russell MRG; Electron Microscopy Science Technology Platform, The Francis Crick Institute, London, United Kingdom.
  • Wagener J; Signalling in Apicomplexan Parasites Laboratory, The Francis Crick Institute, London, United Kingdom.
  • Kent R; Department of Microbiology and Molecular Genetics, University of Vermont Larner College of Medicine, Burlington, United States.
  • Carmeille R; Department of Molecular and Cell Biology, University of Connecticut, Storrs, United States.
  • Peddie CJ; Electron Microscopy Science Technology Platform, The Francis Crick Institute, London, United Kingdom.
  • Collinson L; Electron Microscopy Science Technology Platform, The Francis Crick Institute, London, United Kingdom.
  • Heaslip A; Department of Molecular and Cell Biology, University of Connecticut, Storrs, United States.
  • Ward GE; Department of Microbiology and Molecular Genetics, University of Vermont Larner College of Medicine, Burlington, United States.
  • Treeck M; Signalling in Apicomplexan Parasites Laboratory, The Francis Crick Institute, London, United Kingdom.
Elife ; 82019 10 02.
Article en En | MEDLINE | ID: mdl-31577230
Toxoplasma gondii contains a limited subset of actin binding proteins. Here we show that the putative actin regulator cyclase-associated protein (CAP) is present in two different isoforms and its deletion leads to significant defects in some but not all actin dependent processes. We observe defects in cell-cell communication, daughter cell orientation and the juxtanuclear accumulation of actin, but only modest defects in synchronicity of division and no defect in the replication of the apicoplast. 3D electron microscopy reveals that loss of CAP results in a defect in formation of a normal central residual body, but parasites remain connected within the vacuole. This dissociates synchronicity of division and parasite rosetting and reveals that establishment and maintenance of the residual body may be more complex than previously thought. These results highlight the different spatial requirements for F-actin regulation in Toxoplasma which appear to be achieved by partially overlapping functions of actin regulators.
Asunto(s)
Palabras clave

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Toxoplasma / Proteínas Protozoarias / Actinas Tipo de estudio: Risk_factors_studies Idioma: En Revista: Elife Año: 2019 Tipo del documento: Article País de afiliación: Reino Unido Pais de publicación: Reino Unido

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Toxoplasma / Proteínas Protozoarias / Actinas Tipo de estudio: Risk_factors_studies Idioma: En Revista: Elife Año: 2019 Tipo del documento: Article País de afiliación: Reino Unido Pais de publicación: Reino Unido