Extramacrochaetae promotes branch and bouton number via the sequestration of daughterless in the cytoplasm of neurons.
Dev Neurobiol
; 79(8): 805-818, 2019 08.
Article
en En
| MEDLINE
| ID: mdl-31581354
ABSTRACT
The Class I basic helix-loop-helix (bHLH) proteins are highly conserved transcription factors that are ubiquitously expressed. A wealth of literature on Class I bHLH proteins has shown that these proteins must homodimerize or heterodimerize with tissue-specific HLH proteins in order to bind DNA at E-box consensus sequences to control tissue-specific transcription. Due to its ubiquitous expression, Class I bHLH proteins are also extensively regulated posttranslationally, mostly through dimerization. Previously, we reported that in addition to its role in promoting neurogenesis, the Class I bHLH protein daughterless also functions in mature neurons to restrict axon branching and synapse number. Here, we show that part of the molecular logic that specifies how daughterless functions in neurogenesis is also conserved in neurons. We show that the Type V HLH protein extramacrochaetae (Emc) binds to and represses daughterless function by sequestering daughterless to the cytoplasm. This work provides initial insights into the mechanisms underlying the function of daughterless and Emc in neurons while providing a novel understanding of how Emc functions to restrict daughterless activity within the cell.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Proteínas Represoras
/
Regulación del Desarrollo de la Expresión Génica
/
Citoplasma
/
Proteínas de Drosophila
/
Drosophila melanogaster
/
Factores de Transcripción con Motivo Hélice-Asa-Hélice Básico
/
Neuronas
Límite:
Animals
Idioma:
En
Revista:
Dev Neurobiol
Asunto de la revista:
BIOLOGIA
/
NEUROLOGIA
Año:
2019
Tipo del documento:
Article