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Novel adsorptive materials by adenosine 5'-triphosphate imprinted-polymer over the surface of polystyrene nanospheres for selective separation of adenosine 5'-triphosphate biomarker from urine.
Jadda, Ramana; Madhumanchi, Sreenu; Suedee, Roongnapa.
Afiliación
  • Jadda R; Molecular Recognition Materials Research Unit, Department of Pharmaceutical Chemistry, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Hat Yai, Thailand.
  • Madhumanchi S; Molecular Recognition Materials Research Unit, Department of Pharmaceutical Chemistry, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Hat Yai, Thailand.
  • Suedee R; Molecular Recognition Materials Research Unit, Department of Pharmaceutical Chemistry, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Hat Yai, Thailand.
J Sep Sci ; 42(24): 3662-3678, 2019 Dec.
Article en En | MEDLINE | ID: mdl-31591808
In this study, we have developed a method to assess adenosine 5'-triphosphate by adsorptive extraction using surface adenosine 5'-triphosphate-imprinted polymer over polystyrene nanoparticles (412 ± 16 nm) for selective recognition/separation from urine. Molecularly imprinted polymer was synthesized by emulsion copolymerization reaction using adenosine 5'-triphosphate as a template, functional monomers (methacrylic acid, N-isopropyl acrylamide, and dimethylamino ethylmethacrylate) and a crosslinker, methylenebisacrylamide. The binding capacities of imprinted and non-imprinted polymers were measured using high-performance liquid chromatography with UV detection with a detection limit of 1.6 ± 0.02 µM of adenosine 5'-triphosphate in the urine. High binding affinity (QMIP , 42.65 µmol/g), and high selectivity and specificity to adenosine 5'-triphosphate compared to other competitive nucleotides including adenosine 5'-diphosphate, adenosine 5'-monophosphate, and analogs such as adenosine, adenine, uridine, uric acid, and creatinine were observed. The imprinting efficiency of imprinted polymer is 2.11 for urine (QMIP , 100.3 µmol/g) and 2.51 for synthetic urine (QMIP , 48.5 µmol/g). The extraction protocol was successfully applied to the direct extraction of adenosine 5'-triphosphate from spiked human urine indicating that this synthesized molecularly imprinted polymer allowed adenosine 5'-triphosphate to be preconcentrated while simultaneously interfering compounds were removed from the matrix. These submicron imprinted polymers over nano polystyrene spheres have a potential in the pharmaceutical industries and clinical analysis applications.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Polímeros / Poliestirenos / Adenosina Trifosfato / Impresión Molecular / Nanosferas Tipo de estudio: Guideline Idioma: En Revista: J Sep Sci Año: 2019 Tipo del documento: Article País de afiliación: Tailandia Pais de publicación: Alemania

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Polímeros / Poliestirenos / Adenosina Trifosfato / Impresión Molecular / Nanosferas Tipo de estudio: Guideline Idioma: En Revista: J Sep Sci Año: 2019 Tipo del documento: Article País de afiliación: Tailandia Pais de publicación: Alemania