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Patient-derived organoids can predict response to chemotherapy in metastatic colorectal cancer patients.
Ooft, Salo N; Weeber, Fleur; Dijkstra, Krijn K; McLean, Chelsea M; Kaing, Sovann; van Werkhoven, Erik; Schipper, Luuk; Hoes, Louisa; Vis, Daniel J; van de Haar, Joris; Prevoo, Warner; Snaebjornsson, Petur; van der Velden, Daphne; Klein, Michelle; Chalabi, Myriam; Boot, Henk; van Leerdam, Monique; Bloemendal, Haiko J; Beerepoot, Laurens V; Wessels, Lodewyk; Cuppen, Edwin; Clevers, Hans; Voest, Emile E.
Afiliación
  • Ooft SN; Department of Molecular Oncology and Immunology, Netherlands Cancer Institute, 1066 CX Amsterdam, Netherlands.
  • Weeber F; Oncode Institute, 3521 AL Utrecht, Netherlands.
  • Dijkstra KK; Department of Molecular Oncology and Immunology, Netherlands Cancer Institute, 1066 CX Amsterdam, Netherlands.
  • McLean CM; Oncode Institute, 3521 AL Utrecht, Netherlands.
  • Kaing S; Department of Molecular Oncology and Immunology, Netherlands Cancer Institute, 1066 CX Amsterdam, Netherlands.
  • van Werkhoven E; Oncode Institute, 3521 AL Utrecht, Netherlands.
  • Schipper L; Department of Molecular Oncology and Immunology, Netherlands Cancer Institute, 1066 CX Amsterdam, Netherlands.
  • Hoes L; Oncode Institute, 3521 AL Utrecht, Netherlands.
  • Vis DJ; Department of Molecular Oncology and Immunology, Netherlands Cancer Institute, 1066 CX Amsterdam, Netherlands.
  • van de Haar J; Oncode Institute, 3521 AL Utrecht, Netherlands.
  • Prevoo W; Department of Biometrics, Netherlands Cancer Institute, 1066 CX Amsterdam, Netherlands.
  • Snaebjornsson P; Department of Molecular Oncology and Immunology, Netherlands Cancer Institute, 1066 CX Amsterdam, Netherlands.
  • van der Velden D; Oncode Institute, 3521 AL Utrecht, Netherlands.
  • Klein M; Department of Molecular Oncology and Immunology, Netherlands Cancer Institute, 1066 CX Amsterdam, Netherlands.
  • Chalabi M; Oncode Institute, 3521 AL Utrecht, Netherlands.
  • Boot H; Oncode Institute, 3521 AL Utrecht, Netherlands.
  • van Leerdam M; Department of Molecular Carcinogenesis, Netherlands Cancer Institute, 1066 CX Amsterdam, Netherlands.
  • Bloemendal HJ; Department of Molecular Oncology and Immunology, Netherlands Cancer Institute, 1066 CX Amsterdam, Netherlands.
  • Beerepoot LV; Oncode Institute, 3521 AL Utrecht, Netherlands.
  • Wessels L; Department of Molecular Carcinogenesis, Netherlands Cancer Institute, 1066 CX Amsterdam, Netherlands.
  • Cuppen E; Department of Radiology, Netherlands Cancer Institute, 1066 CX Amsterdam, Netherlands.
  • Clevers H; Department of Pathology, Netherlands Cancer Institute, 1066 CX Amsterdam, Netherlands.
  • Voest EE; Department of Molecular Oncology and Immunology, Netherlands Cancer Institute, 1066 CX Amsterdam, Netherlands.
Sci Transl Med ; 11(513)2019 10 09.
Article en En | MEDLINE | ID: mdl-31597751
There is a clear and unmet clinical need for biomarkers to predict responsiveness to chemotherapy for cancer. We developed an in vitro test based on patient-derived tumor organoids (PDOs) from metastatic lesions to identify nonresponders to standard-of-care chemotherapy in colorectal cancer (CRC). In a prospective clinical study, we show the feasibility of generating and testing PDOs for evaluation of sensitivity to chemotherapy. Our PDO test predicted response of the biopsied lesion in more than 80% of patients treated with irinotecan-based therapies without misclassifying patients who would have benefited from treatment. This correlation was specific to irinotecan-based chemotherapy, however, and the PDOs failed to predict outcome for treatment with 5-fluorouracil plus oxaliplatin. Our data suggest that PDOs could be used to prevent cancer patients from undergoing ineffective irinotecan-based chemotherapy.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias Colorrectales / Organoides Tipo de estudio: Observational_studies / Prognostic_studies / Risk_factors_studies Límite: Female / Humans Idioma: En Revista: Sci Transl Med Asunto de la revista: CIENCIA / MEDICINA Año: 2019 Tipo del documento: Article País de afiliación: Países Bajos Pais de publicación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias Colorrectales / Organoides Tipo de estudio: Observational_studies / Prognostic_studies / Risk_factors_studies Límite: Female / Humans Idioma: En Revista: Sci Transl Med Asunto de la revista: CIENCIA / MEDICINA Año: 2019 Tipo del documento: Article País de afiliación: Países Bajos Pais de publicación: Estados Unidos