Quantitative study of doxorubicin in living cell nuclei by microspectrofluorometry.
Biochim Biophys Acta
; 950(1): 13-20, 1988 May 06.
Article
en En
| MEDLINE
| ID: mdl-3162810
ABSTRACT
Doxorubicin-DNA association has been studied by quantitative microspectrofluorometry. Fluorescence emission spectra from a microvolume of single living cell nuclei treated with doxorubicin have been analyzed in terms of difference in spectral shape and fluorescence yield between free and DNA-bound drug. Contribution of each spectral component to the total signal was calculated by least-squares linear regression. With this method of analysis, total drug concentration has been determined with an error of less than 10%. Moreover, the uptake into the nucleus has been studied in a non destructive way, avoiding use of 14C-labelled drug. Kinetic studies of drug accumulation into the nuclei were conducted on sensitive and resistant cells.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Doxorrubicina
/
Núcleo Celular
Límite:
Humans
Idioma:
En
Revista:
Biochim Biophys Acta
Año:
1988
Tipo del documento:
Article
País de afiliación:
Francia