Biological insights into non-model microbial hosts through stable-isotope metabolic flux analysis.

ABSTRACT

Stable-isotope metabolic flux analysis is an important approach to unravel the metabolic network and its regulation in organisms. It has become a key analytical technology for biotechnological applications. During recent years non-model microorganisms have received increasing attention because they possess unique metabolic capabilities and can serve as a host for production of biofuels and biochemicals. Stable-isotope metabolic flux analysis has been widely used in these microorganisms for exploring novel pathways, elucidating the operation of central metabolic networks, and revealing the metabolic changes that result from genetic manipulations. Here, we review recent applications of stable-isotope metabolic flux analysis in characterizing non-model microbial hosts, guiding the development of rational engineering strategies for enhancement of biochemical production and extension of substrate range, and understanding of industrial production processes.