Molecular Detection of Human Salivirus in Italy Through Monitoring of Urban Sewages.
Food Environ Virol
; 12(1): 68-74, 2020 03.
Article
en En
| MEDLINE
| ID: mdl-31641938
ABSTRACT
Salivirus (SalV) is a newly discovered virus associated to acute gastroenteritis in humans. In Italy, its prevalence and genetic diversity is unknown. To reduce this knowledge gap, 124 sewage samples collected throughout the country were analyzed for SalV by two nested RT-PCRs targeting the 5'UTR and the 3D regions and by real-time RT-qPCR. Virus RNA was detected in 37 (29.8%) samples; of these, 24 could be characterized and all belonged to genotype A1. Viral concentrations ranged between 2.8 × 103 and 1.9 × 105 genome copies per liter. This is the first report of SalV occurrence in water environments in Italy, suggesting that SalV infection is not uncommon in this country.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Picornaviridae
/
Aguas del Alcantarillado
/
Infecciones por Picornaviridae
/
Gastroenteritis
Tipo de estudio:
Diagnostic_studies
/
Risk_factors_studies
Límite:
Humans
País/Región como asunto:
Europa
Idioma:
En
Revista:
Food Environ Virol
Año:
2020
Tipo del documento:
Article
País de afiliación:
Italia