MicroRNA-665 mediates propofol-induced cell apoptosis in human stem cell-derived neurons.
Bioengineered
; 10(1): 493-500, 2019 12.
Article
en En
| MEDLINE
| ID: mdl-31656124
ABSTRACT
We aimed to evaluate the neurotoxicity and mechanisms of anesthetics propofol in hESC-derived neurons. Cell apoptosis in hESC-derived neurons' exposure to 4, 10 and 20 µg/mL propofol for 6 h was assessed using terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate in situ nick end labeling (TUNEL) staining and microRNA-665 (miR-665) expression was assessed using quantitative reverse transcription polymerase chain reaction (qRT-PCR). miR-665 was overexpressed and knocked down using a miR-665 mimic and anti-665 transfection, respectively. The results showed that hESCs exposed to propofol showed a dose-dependent cell apoptosis, followed by the upregulation of miR-665 expression. Overexpression of miR-665 increased propofol-induced apoptosis in hESC cells. And targeting miR-665 decreased propofol-induced cell apoptosis in hESC cells. These data suggest that propofol induces cell death in hESC-derived neurons and the propofol-induced cell apoptosis may occur via miR-665-dependent mechanism.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Células Madre
/
Propofol
/
Apoptosis
/
Anestésicos Intravenosos
/
MicroARNs
/
Neuronas
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Bioengineered
Año:
2019
Tipo del documento:
Article
País de afiliación:
China