HPLC-UV and spectrofluorimetric methods for simultaneous estimation of fluticasone furoate and vilanterol in rabbit plasma: A pharmacokinetic study.
J Chromatogr B Analyt Technol Biomed Life Sci
; 1132: 121842, 2019 Nov 15.
Article
en En
| MEDLINE
| ID: mdl-31669821
ABSTRACT
Fluticasone furoate (FF) and vilanterol trifenatate (VT) is a widely prescribed combination in the management of asthma and chronic obstructive pulmonary disease. In the present study, two quantitative methods based on HPLC-UV and spectrofluorimetric analysis had been developed and validated for simultaneous estimation of FF and VT in rabbit plasma using baclomethasone as internal standard (ISTD). Analytes and ISTD were separated from plasma using simple step of protein precipitation with acetonitrile. Chromatographic separation was achieved on Spherisorb S5 ODS2 (250â¯mmâ¯×â¯4.6â¯mm, 5.0⯵m) column using mobile phase that constitute acetonitrile-0.01% glacial acetic acid in water (7030, v/v) and then detected on a UV detector at 235â¯nm wavelength. Spectrofluorimetric detection was performed using absorption/emission wavelength (λabs/em) of 286/352â¯nm and 362/407â¯nm for FF and VT, respectively. For both analytes, linearity ranged from 4-200â¯ng/mL to 10-200â¯ng/mL using HPLC-UV and spectrofluorimetric method, respectively. Methods were validated as per FDA recommendations. Statistical analysis revealed that these detection methods are statistically insignificant difference and can be used interchangeably without any bias. Further, these methods were applied in pharmacokinetic study for simultaneous estimation of FF and VT in rabbit plasma.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Alcoholes Bencílicos
/
Clorobencenos
/
Cromatografía Líquida de Alta Presión
/
Androstadienos
Tipo de estudio:
Diagnostic_studies
/
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
J Chromatogr B Analyt Technol Biomed Life Sci
Asunto de la revista:
ENGENHARIA BIOMEDICA
Año:
2019
Tipo del documento:
Article
País de afiliación:
India