Characterization of the direct interaction between apple condensed tannins and cholesterol in vitro.
Food Chem
; 309: 125762, 2020 Mar 30.
Article
en En
| MEDLINE
| ID: mdl-31670123
ABSTRACT
To provide the scientific evidences for a possible new hypocholesterolemic mechanism of apple condensed tannins (ACT), the direct interaction of ACT with cholesterol (CH) was investigated in the present study. Our results suggested that the quenching of ACT fluorescence by CH was carried out according to a static mechanism, while the interaction between ACT and CH in vitro was a spontaneous process. ACT were capable of binding with CH directly, and the CH-binding capacity (35.9-43.9%) of ACT remarkably enhanced with the increase of ACT concentration (0.5-2.0â¯mg proanthocyanidin B2 equivalent/mL). Besides, spectroscopic methods and morphological analysis were used to characterize the ACT-CH coprecipitates, the findings indicated that ACT were able to precipitate CH via ionic interactions, hydrophobic interactions and intermolecular hydrogen bonds rather than covalent bonds. In conclusion, the direct interaction of ACT with CH might play a role in their CH-lowering effects in humans and animals.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Taninos
/
Colesterol
/
Malus
Idioma:
En
Revista:
Food Chem
Año:
2020
Tipo del documento:
Article