Porcine IVF embryo development and estrogen receptors are influenced by the concentration of percoll gradients during sperm selection.
Mol Reprod Dev
; 87(1): 135-141, 2020 01.
Article
en En
| MEDLINE
| ID: mdl-31691431
ABSTRACT
This study aimed to evaluate the effect of three different concentrations of discontinuous gradients of percoll (90/45, 80/40, and 70/35) in the outcome of porcine in vitro fertilization (IVF) and its influence on further embryo development and quality. Embryo viability was assessed by the expression of estrogen receptors (E2 R) and cleaved caspase-3 (CC3). The highest percoll concentration (90/45) resulted in the lowest embryo production (24.9%) in comparison with 80/40 (37.5%) and 70/35 (40.0%), with the production being similar between the two lowest concentrations. The hatching rate for 90/45 (26.2%) was lower than for 80/40 (45.5%), and both were similar to the Group 70/35 (32.9%). The hatched embryos from the concentration 90/45 showed the lowest proportion of E2 R expression (3.6%), while the Groups 80/40 (22.6%) and 70/35 (39.3%) had a similar proportion of expression. The live embryos that did not hatch until Day 8 of culture presented a higher CC3 proportion for Group 90/45 (18.3%), in comparison with 80/40 (12.7%) and 70/35 (10.7%), with the latter two being similar. In conclusion, adjustments in percoll concentration used for sperm selection before porcine IVF can improve embryo production and competence for pregnancy recognition and establishment.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Espermatozoides
/
Porcinos
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Transducción de Señal
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Fertilización In Vitro
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Receptores de Estrógenos
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Povidona
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Dióxido de Silicio
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Desarrollo Embrionario
Límite:
Animals
Idioma:
En
Revista:
Mol Reprod Dev
Asunto de la revista:
BIOLOGIA MOLECULAR
/
MEDICINA REPRODUTIVA
Año:
2020
Tipo del documento:
Article
País de afiliación:
Brasil