Suppression of Carnosine on Adhesion and Extravasation of Human Colorectal Cancer Cells.
Anticancer Res
; 39(11): 6135-6144, 2019 Nov.
Article
en En
| MEDLINE
| ID: mdl-31704841
ABSTRACT
AIM:
To investigate the effect of carnosine, an active compound of dietary beef, fish and chicken, on the regulation of cell adhesion and extravasation during metastasis. MATERIALS ANDMETHODS:
Cell adhesion and extravasation abilities, and related regulating molecular mechanisms were analyzed in human colorectal cancer cells (HCT-116) and human umbilical vein cells (EA.hy926).RESULTS:
Carnosine reduced the ability of HCT-116 cells to adhere to EA.hy926 cells. The expression levels of integrin-ß1 in HCT-116 cells, as well as of intercellular adhesion molecule-1 and E-selectin in EA.hy926 cells, were reduced after carnosine treatment. After EA.hy926 cells were treated with carnosine, phosphorylation of vascular endothelia-cadherin (VE-cadherin), protein levels of Ras homologous (RHO) and RHO-associated coiled-coil containing protein kinase, and levels of reactive oxygen species were reduced. After treating EA.hy926 cells with carnosine, phosphorylation of inhibitor of kappa B (IκB) and DNA binding activity of nuclear factor-κB (NF-κB) were reduced.CONCLUSION:
Carnosine inhibits metastatic cell adhesion and extravasation by suppressing NF-κB signaling activation.Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Carnosina
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Neoplasias Colorrectales
/
Regulación Neoplásica de la Expresión Génica
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Adhesión Celular
/
FN-kappa B
Límite:
Humans
Idioma:
En
Revista:
Anticancer Res
Año:
2019
Tipo del documento:
Article