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Induction of the germ cell fate from pluripotent stem cells in cynomolgus monkeys†.
Sakai, Yoshitake; Nakamura, Tomonori; Okamoto, Ikuhiro; Gyobu-Motani, Sayuri; Ohta, Hiroshi; Yabuta, Yukihiro; Tsukiyama, Tomoyuki; Iwatani, Chiduru; Tsuchiya, Hideaki; Ema, Masatsugu; Morizane, Asuka; Takahashi, Jun; Yamamoto, Takuya; Saitou, Mitinori.
Afiliación
  • Sakai Y; Institute for the Advanced Study of Human Biology (ASHBi), Kyoto University, Kyoto, Japan.
  • Nakamura T; Department of Anatomy and Cell Biology, Graduate School of Medicine, Kyoto University, Kyoto, Japan.
  • Okamoto I; Institute for the Advanced Study of Human Biology (ASHBi), Kyoto University, Kyoto, Japan.
  • Gyobu-Motani S; Department of Anatomy and Cell Biology, Graduate School of Medicine, Kyoto University, Kyoto, Japan.
  • Ohta H; Institute for the Advanced Study of Human Biology (ASHBi), Kyoto University, Kyoto, Japan.
  • Yabuta Y; Department of Anatomy and Cell Biology, Graduate School of Medicine, Kyoto University, Kyoto, Japan.
  • Tsukiyama T; Institute for the Advanced Study of Human Biology (ASHBi), Kyoto University, Kyoto, Japan.
  • Iwatani C; Department of Anatomy and Cell Biology, Graduate School of Medicine, Kyoto University, Kyoto, Japan.
  • Tsuchiya H; Institute for the Advanced Study of Human Biology (ASHBi), Kyoto University, Kyoto, Japan.
  • Ema M; Department of Anatomy and Cell Biology, Graduate School of Medicine, Kyoto University, Kyoto, Japan.
  • Morizane A; Institute for the Advanced Study of Human Biology (ASHBi), Kyoto University, Kyoto, Japan.
  • Takahashi J; Department of Anatomy and Cell Biology, Graduate School of Medicine, Kyoto University, Kyoto, Japan.
  • Yamamoto T; Institute for the Advanced Study of Human Biology (ASHBi), Kyoto University, Kyoto, Japan.
  • Saitou M; Research Center for Animal Life Science, Shiga University of Medical Science, Shiga, Japan.
Biol Reprod ; 102(3): 620-638, 2020 03 13.
Article en En | MEDLINE | ID: mdl-31724030
In vitro reconstitution of germ-cell development from pluripotent stem cells (PSCs) has created key opportunities to explore the fundamental mechanisms underlying germ-cell development, particularly in mice and humans. Importantly, such investigations have clarified critical species differences in the mechanisms regulating mouse and human germ-cell development, highlighting the necessity of establishing an in vitro germ-cell development system in other mammals, such as non-human primates. Here, we show that multiple lines of embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) in cynomolgus monkeys (Macaca fascicularis; cy) can be maintained stably in an undifferentiated state under a defined condition with an inhibitor for WNT signaling, and such PSCs are induced efficiently into primordial germ cell-like cells (PGCLCs) bearing a transcriptome similar to early cyPGCs. Interestingly, the induction kinetics of cyPGCLCs from cyPSCs is faster than that of human (h) PGCLCs from hPSCs, and while the transcriptome dynamics during cyPGCLC induction is relatively similar to that during hPGCLC induction, it is substantially divergent from that during mouse (m) PGCLC induction. Our findings delineate common as well as species-specific traits for PGC specification, creating a foundation for parallel investigations into the mechanism for germ-cell development in mice, monkeys, and humans.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Diferenciación Celular / Células Madre Pluripotentes Límite: Animals Idioma: En Revista: Biol Reprod Año: 2020 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Diferenciación Celular / Células Madre Pluripotentes Límite: Animals Idioma: En Revista: Biol Reprod Año: 2020 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Estados Unidos