Direct Labeling of Protein Nanoparticles with Fluorescent Compounds for Immunoassay Applications.
Anal Sci
; 36(3): 385-387, 2020 Mar 10.
Article
en En
| MEDLINE
| ID: mdl-31735760
ABSTRACT
A fusion protein, designated ELP-D-C, comprised of a hydrophobic elastin-like polypeptide unit, a hydrophilic aspartic acid-rich peptide unit, and an antibody-binding domain as a functional unit, was constructed. Upon heat induction, ELP-D-C forms micellar nanoparticles displaying antibody-binding domains on their surfaces. The protein nanoparticles were able to incorporate hydrophobic fluorescent compounds and subsequently detect target molecules via antibody binding by the resulting fluorescence intensity, which was proportional to the log of the concentration of the target molecule.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Inmunoensayo
/
Nanopartículas
/
Colorantes Fluorescentes
Idioma:
En
Revista:
Anal Sci
Año:
2020
Tipo del documento:
Article
País de afiliación:
Japón