Tuning pore features of mineralized collagen/PCL scaffolds for cranial bone regeneration in a rat model.
Mater Sci Eng C Mater Biol Appl
; 106: 110186, 2020 Jan.
Article
en En
| MEDLINE
| ID: mdl-31753406
ABSTRACT
Porosity is indispensable for a bone tissue-engineered scaffold for facilitating endogenous cell migration and nascent bone ingrowth. In large-sized cranial bone defect repair, porous scaffolds meet great challenges to match cranial bone regeneration and provide sufficient protection with structural integrity. Therefore, the pore features of the scaffolds for cranial bone regeneration should differ from those typical porous scaffolds used in tubular bone repair and be finely tuned. In this study, a series of porous mineralized collagen/PCL scaffolds with different pore features were fabricated via freeze-drying and applied in a Sprague Dawley rat cranial bone calvarial defect model. The pore size for four groups increased from 10-45⯵m to 40-130⯵m. As scaffold porosity increased, the compressive strength decreased from 2.09⯱â¯0.12â¯MPa to 0.51⯱â¯0.04â¯MPa. The micro-computed tomography three-dimensional reconstruction images showed that as pore size and porosity increased, the amount of new bone formation had a maximum in group 3 (pore size 20-100⯵m, compressive strength 1.06⯱â¯0.03â¯MPa). In addition, the histological and histomorphometric analyses showed a consistent tendency which confirmed the Micro-CT results. Meanwhile, histological findings including bony bridging, tissue response at the bone-implant interface and fibrous capsule thickness indicated that the dura mater pathway played the most important role in the regenerative process of this calvarial defect model.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Cráneo
/
Colágeno
/
Andamios del Tejido
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
Mater Sci Eng C Mater Biol Appl
Año:
2020
Tipo del documento:
Article
País de afiliación:
China