A LC-MS/MS method for the quantification of caffeine, betamethasone, clonidine and furosemide in cerebrospinal fluid of preterm infants.
J Pharm Biomed Anal
; 179: 112996, 2020 Feb 05.
Article
en En
| MEDLINE
| ID: mdl-31767227
ABSTRACT
BACKGROUND:
Newborns, admitted to the Neonatal Intensive Care Unit (NICU), are exposed to a large number of medications, the majority of which are not labeled for use in infants, especially in preterm newborns, because clinical trials on their benefits and harms are lacking. There is a huge gap in knowledge on pharmacokinetic (PK) data in sick preterm infants, including that of drug penetration to cerebrospinal fluid (CSF). One of the issues is related to the lack of reliable analytical methods for the measurement of drugs in CSF.METHODS:
In this paper we describe a specific and sensitive LC-MS/MS method for the simultaneous quantification in CSF of four commonly prescribed drugs in NICUs caffeine, betamethasone, clonidine and furosemide.RESULTS:
The method was validated following EMA guidelines and applied to several CSF samples of preterm infants with post-hemorrhagic ventricular dilatation in which a ventricular access device was applied. The range of the concentrations of the four drugs measured in the CSF was wide.CONCLUSIONS:
Our method can be considered useful for further clinical studies to describe the PK aspects of these drugs in neonatal medicine.Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Cromatografía Liquida
/
Espectrometría de Masas en Tándem
Tipo de estudio:
Guideline
Límite:
Female
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Humans
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Male
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Newborn
Idioma:
En
Revista:
J Pharm Biomed Anal
Año:
2020
Tipo del documento:
Article
País de afiliación:
Suecia