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Trans-omics Impact of Thymoproteasome in Cortical Thymic Epithelial Cells.
Ohigashi, Izumi; Tanaka, Yu; Kondo, Kenta; Fujimori, Sayumi; Kondo, Hiroyuki; Palin, Amy C; Hoffmann, Victoria; Kozai, Mina; Matsushita, Yosuke; Uda, Shinsuke; Motosugi, Ryo; Hamazaki, Jun; Kubota, Hiroyuki; Murata, Shigeo; Tanaka, Keiji; Katagiri, Toyomasa; Kosako, Hidetaka; Takahama, Yousuke.
Afiliación
  • Ohigashi I; Division of Experimental Immunology, Institute of Advanced Medical Sciences, University of Tokushima, Tokushima 770-8503, Japan.
  • Tanaka Y; Experimental Immunology Branch, National Cancer Institute, NIH, Bethesda, MD 20892, USA.
  • Kondo K; Experimental Immunology Branch, National Cancer Institute, NIH, Bethesda, MD 20892, USA.
  • Fujimori S; Division of Experimental Immunology, Institute of Advanced Medical Sciences, University of Tokushima, Tokushima 770-8503, Japan.
  • Kondo H; Division of Experimental Immunology, Institute of Advanced Medical Sciences, University of Tokushima, Tokushima 770-8503, Japan.
  • Palin AC; Experimental Immunology Branch, National Cancer Institute, NIH, Bethesda, MD 20892, USA.
  • Hoffmann V; Division of Veterinary Resources, Office of Research Services, NIH, Bethesda, MD 20892, USA.
  • Kozai M; Division of Experimental Immunology, Institute of Advanced Medical Sciences, University of Tokushima, Tokushima 770-8503, Japan.
  • Matsushita Y; Division of Genome Medicine, Institute of Advanced Medical Sciences, University of Tokushima, Tokushima 770-8503, Japan.
  • Uda S; Division of Integrated Omics, Medical Institute of Bioregulation, Kyushu University, Fukuoka 812-8582, Japan.
  • Motosugi R; Laboratory of Protein Metabolism, Graduate School of Pharmaceutical Sciences, The University of Tokyo, Tokyo 113-0033, Japan.
  • Hamazaki J; Laboratory of Protein Metabolism, Graduate School of Pharmaceutical Sciences, The University of Tokyo, Tokyo 113-0033, Japan.
  • Kubota H; Division of Integrated Omics, Medical Institute of Bioregulation, Kyushu University, Fukuoka 812-8582, Japan.
  • Murata S; Laboratory of Protein Metabolism, Graduate School of Pharmaceutical Sciences, The University of Tokyo, Tokyo 113-0033, Japan.
  • Tanaka K; Tokyo Metropolitan Institute for Medical Science, Tokyo 156-8506, Japan.
  • Katagiri T; Division of Genome Medicine, Institute of Advanced Medical Sciences, University of Tokushima, Tokushima 770-8503, Japan.
  • Kosako H; Division of Cell Signaling, Fujii Memorial Institute of Medical Sciences, Institute of Advanced Medical Sciences, University of Tokushima, Tokushima 770-8503, Japan.
  • Takahama Y; Experimental Immunology Branch, National Cancer Institute, NIH, Bethesda, MD 20892, USA. Electronic address: yousuke.takahama@nih.gov.
Cell Rep ; 29(9): 2901-2916.e6, 2019 11 26.
Article en En | MEDLINE | ID: mdl-31775054
ABSTRACT
The thymic function to produce self-protective and self-tolerant T cells is chiefly mediated by cortical thymic epithelial cells (cTECs) and medullary TECs (mTECs). Recent studies including single-cell transcriptomic analyses have highlighted a rich diversity in functional mTEC subpopulations. Because of their limited cellularity, however, the biochemical characterization of TECs, including the proteomic profiling of cTECs and mTECs, has remained unestablished. Utilizing genetically modified mice that carry enlarged but functional thymuses, here we show a combination of proteomic and transcriptomic profiles for cTECs and mTECs, which identified signature molecules that characterize a developmental and functional contrast between cTECs and mTECs. Our results reveal a highly specific impact of the thymoproteasome on proteasome subunit composition in cTECs and provide an integrated trans-omics platform for further exploration of thymus biology.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Timo / Proteómica / Células Epiteliales Límite: Humans Idioma: En Revista: Cell Rep Año: 2019 Tipo del documento: Article País de afiliación: Japón
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Timo / Proteómica / Células Epiteliales Límite: Humans Idioma: En Revista: Cell Rep Año: 2019 Tipo del documento: Article País de afiliación: Japón