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Utilizing Stimulated Raman Scattering Microscopy To Study Intracellular Distribution of Label-Free Ponatinib in Live Cells.
Sepp, Kristel; Lee, Martin; Bluntzer, Marie T J; Helgason, G Vignir; Hulme, Alison N; Brunton, Valerie G.
Afiliación
  • Sepp K; Edinburgh Cancer Research UK Centre, Institute of Genetics & Molecular Medicine, University of Edinburgh, Edinburgh EH4 2XR, U.K.
  • Lee M; EaStCHEM School of Chemistry, University of Edinburgh, Joseph Black Building, David Brewster Road, Edinburgh EH9 3FJ, U.K.
  • Bluntzer MTJ; Edinburgh Cancer Research UK Centre, Institute of Genetics & Molecular Medicine, University of Edinburgh, Edinburgh EH4 2XR, U.K.
  • Helgason GV; EaStCHEM School of Chemistry, University of Edinburgh, Joseph Black Building, David Brewster Road, Edinburgh EH9 3FJ, U.K.
  • Hulme AN; Wolfson Wohl Cancer Research Centre, Institute of Cancer Sciences, University of Glasgow, Garscube Estate, Glasgow G61 1QH, U.K.
  • Brunton VG; EaStCHEM School of Chemistry, University of Edinburgh, Joseph Black Building, David Brewster Road, Edinburgh EH9 3FJ, U.K.
J Med Chem ; 63(5): 2028-2034, 2020 03 12.
Article en En | MEDLINE | ID: mdl-31829628
ABSTRACT
Stimulated Raman scattering (SRS) microscopy represents a powerful method for imaging label-free drug distribution with high resolution. SRS was applied to image label-free ponatinib with high sensitivity and specificity in live human chronic myeloid leukemia (CML) cell lines. This was achieved at biologically relevant, nanomolar concentrations, allowing determination of ponatinib uptake and sequestration into lysosomes during the development of acquired drug resistance and an improved understanding of target engagement.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Piridazinas / Microscopía Óptica no Lineal / Imidazoles / Líquido Intracelular / Antineoplásicos Límite: Humans Idioma: En Revista: J Med Chem Asunto de la revista: QUIMICA Año: 2020 Tipo del documento: Article País de afiliación: Reino Unido
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Piridazinas / Microscopía Óptica no Lineal / Imidazoles / Líquido Intracelular / Antineoplásicos Límite: Humans Idioma: En Revista: J Med Chem Asunto de la revista: QUIMICA Año: 2020 Tipo del documento: Article País de afiliación: Reino Unido