Nanopore sequencing reveals a structural alteration of mirror-image duplicated genes in a genome-editing mouse line.
Congenit Anom (Kyoto)
; 60(4): 120-125, 2020 Jul.
Article
en En
| MEDLINE
| ID: mdl-31837184
ABSTRACT
CRISPR-Cas9 technology has been used in various studies; however, it has also been found to introduce unexpected structural alternations. In this study, we used nanopore sequencing to characterize an unexpected structural alteration of mirror-image duplicated genes in a mouse line, in which we aimed to delete a part of the duplicated genes using genome editing. We removed low-molecular-weight DNA fragments and increased the input, which led to improved sequence performance. With 14.9 Gb input for whole-genome analysis, we detected a complex structural alteration involving inversion and deletion, which appears to be difficult to characterize with short-read sequencers. Therefore, our study clearly showed the utility of nanopore sequencing for characterizing unexpected complex structural alterations caused by genome editing.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Duplicación de Gen
/
Sistemas CRISPR-Cas
/
Edición Génica
/
Secuenciación de Nanoporos
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Congenit Anom (Kyoto)
Asunto de la revista:
TERATOLOGIA
Año:
2020
Tipo del documento:
Article
País de afiliación:
Japón