Single amino acid mutation altered substrate specificity for L-glucose and inositol in <i>scyllo</i>-inositol dehydrogenase isolated from <i>Paracoccus laeviglucosivorans</i>.

Suzuki, Mayu; Koubara, Kairi; Takenoya, Mihoko; Fukano, Kazuhiro; Ito, Shinsaku; Sasaki, Yasuyuki; Nakamura, Akira; Yajima, Shunsuke

Single amino acid mutation altered substrate specificity for L-glucose and inositol in scyllo-inositol dehydrogenase isolated from Paracoccus laeviglucosivorans.

Suzuki, Mayu; Koubara, Kairi; Takenoya, Mihoko; Fukano, Kazuhiro; Ito, Shinsaku; Sasaki, Yasuyuki; Nakamura, Akira; Yajima, Shunsuke.

Afiliación

Suzuki M; Department of Bioscience, Tokyo University of Agriculture, Tokyo, Japan.
Koubara K; Department of Bioscience, Tokyo University of Agriculture, Tokyo, Japan.
Takenoya M; Department of Bioscience, Tokyo University of Agriculture, Tokyo, Japan.
Fukano K; Department of Bioscience, Tokyo University of Agriculture, Tokyo, Japan.
Ito S; Department of Bioscience, Tokyo University of Agriculture, Tokyo, Japan.
Sasaki Y; Department of Bioscience, Tokyo University of Agriculture, Tokyo, Japan.
Nakamura A; Faculty of Life and Environmental Sciences, University of Tsukuba, Ibaraki, Japan.
Yajima S; Microbiology Research Center for Sustainability (MiCS), University of Tsukuba, Ibaraki, Japan.

Biosci Biotechnol Biochem ; 84(4): 734-742, 2020 Apr.

Article en En | MEDLINE | ID: mdl-31842701

Asunto(s)

Sustitución de Aminoácidos; Glucosa/metabolismo; Inositol/metabolismo; Oxidorreductasas/metabolismo; Paracoccus/enzimología; Cinética; Oxidorreductasas/aislamiento & purificación; Conformación Proteica; Especificidad por Sustrato

Palabras clave

Chiral conformation; X-ray crystallography; enzyme kinetics; sugar metabolism

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Oxidorreductasas / Paracoccus / Sustitución de Aminoácidos / Glucosa / Inositol Idioma: En Revista: Biosci Biotechnol Biochem Asunto de la revista: BIOQUIMICA / BIOTECNOLOGIA Año: 2020 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Reino Unido

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