Long non-coding RNA zinc finger antisense 1 expression associates with increased disease risk, elevated disease severity and higher inflammatory cytokines levels in patients with lumbar disc degeneration.
Medicine (Baltimore)
; 98(52): e18465, 2019 Dec.
Article
en En
| MEDLINE
| ID: mdl-31876730
ABSTRACT
This study aimed to investigate the correlation of long noncoding RNA zinc finger antisense 1 (lncRNA ZFAS1) expression with disease risk, disease severity and inflammatory cytokines levels in lumbar disc degeneration (LDD) patients.83 LDD patients underwent surgery and 28 traumatized, non-LDD patients underwent lumbar disc surgery (controls) were consecutively enrolled in this case-control study. Lumbar disc tissue was obtained during surgery and herniated nucleus pulposus (HNP) was isolated to detect lncRNA ZFAS1 expression and inflammatory cytokines mRNA levels by RT-qPCR, and determine protein levels of inflammatory cytokines by western blot.HNP lncRNA ZFAS1 expression in LDD patients was up-regulated compared with controls (Pâ<â.001), and receiver operating characteristic (ROC) curve showed lncRNA ZFAS1 expression disclosed a good predictive value for LDD risk with area under curve (AUC) 0.753 (95% CI 0.646-0.859). And after adjustment by age, gender and body mass index (BMI), lncRNA ZFAS1 (Pâ=â.017) remained to be an independent predictive factor for higher LDD risk. In addition, lncRNA ZFAS1 expression was positively associated with Modified Pfirrmann Grade (Pâ=â.015). As to inflammatory cytokines, lncRNA ZFAS1 expression was observed to be positively correlated with TNF-α (Pâ=â.002), IL-1ß (Pâ=â.007) and IL-6 (Pâ=â.015) mRNAs expressions while reversely associated with IL-10 mRNA level (Pâ=â.014); and lncRNA ZFAS1 expression was also positively correlated with protein levels of TNF-α (Pâ=â.038) and IL-6 (Pâ=â.027) while reversely associated with IL-10 protein expression (Pâ=â.039).lncRNA ZFAS1 expression associates with increased risk, elevated disease severity and higher inflammatory cytokines levels in LDD patients.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Citocinas
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ARN sin Sentido
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Degeneración del Disco Intervertebral
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ARN Largo no Codificante
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Disco Intervertebral
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Desplazamiento del Disco Intervertebral
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Vértebras Lumbares
Tipo de estudio:
Etiology_studies
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Observational_studies
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Prognostic_studies
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Risk_factors_studies
Límite:
Adult
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Female
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Humans
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Male
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Middle aged
Idioma:
En
Revista:
Medicine (Baltimore)
Año:
2019
Tipo del documento:
Article