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Molecular detection of avian spirochete Borrelia anserina in Argas persicus ticks in Algeria.
Ouchene, Nassim; Nebbak, Amira; Ouchene-Khelifi, Nadjet Amina; Dahmani, Ali; Zeroual, Fayçal; Khelef, Djamel; Bitam, Idir; Benakhla, Ahmed; Parola, Philippe.
Afiliación
  • Ouchene N; Institut des Sciences Vétérinaires, Université SAAD Dahlab-Blida1, 09000 Blida, Algeria.
  • Nebbak A; Centre de Recherche Scientifique et Technique en Analyses Physico-Chimiques (CRAPC), Bou Ismail, Tipaza, Algeria.
  • Ouchene-Khelifi NA; Institut des Sciences Vétérinaires, Université SAAD Dahlab-Blida1, 09000 Blida, Algeria.
  • Dahmani A; Institut des Sciences Vétérinaires, Université SAAD Dahlab-Blida1, 09000 Blida, Algeria.
  • Zeroual F; Département des Sciences Vétérinaires, Université Chadli Bendjedid El Tarf, 36000 El Tarf, BP 73, Algeria.
  • Khelef D; Ecole Nationale Supérieure Vétérinaire, Rue Issad Abbes, Oued Smar, Alger, Algeria.
  • Bitam I; Aix Marseille Univ, IRD, AP-HM, SSA, IHU Méditerranée Infection, VITROME, Marseille, France; Ecole Supérieure en Sciences de l'Aliment et des Industries Agroalimentaires (ESSAIA), El Harrach, Alger, Algeria.
  • Benakhla A; Département des Sciences Vétérinaires, Université Chadli Bendjedid El Tarf, 36000 El Tarf, BP 73, Algeria.
  • Parola P; Aix Marseille Univ, IRD, AP-HM, SSA, IHU Méditerranée Infection, VITROME, Marseille, France. Electronic address: philippe.parola@univ-amu.fr.
Comp Immunol Microbiol Infect Dis ; 68: 101408, 2020 Feb.
Article en En | MEDLINE | ID: mdl-31896047
Argasid ticks are one of the most important poultry ectoparasites. They affect poultry directly through blood meal and indirectly through the transmission of pathogens essentially Borrelia anserina, agent of avian borreliosis, one of the most widespread poultry diseases in the world, and is of great economic importance. This study was conducted between April 2014 and March 2015 in the region of Ksar El Boukhari, Algeria, in order to investigate the presence of soft ticks in laying hen farms and to detect B. anserina bacteria using molecular tools. DNA was extracted and screened for the presence of Borrelia spp. DNA by real-time polymerase chain reaction (qPCR). Borrelia spp. screening was performed using primers and probe targeting the 16S rRNA gene. A total of 83 traditional laying hen farms were visited, of which 39 (46.98 %) were found infested with A. persicus tick. Molecular analysis revealed that 2/34 (5.88 %) of ticks were infected by B. anserina. None of the ticks tested were positive for Rickettsia spp., and Coxiella burnetii. These results constitute the first report in Algeria of A. persicus harboring B. anserina.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Enfermedades de las Aves de Corral / Infestaciones por Garrapatas / Borrelia / Argas Tipo de estudio: Diagnostic_studies Límite: Animals País/Región como asunto: Africa Idioma: En Revista: Comp Immunol Microbiol Infect Dis Año: 2020 Tipo del documento: Article País de afiliación: Argelia Pais de publicación: Reino Unido

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Enfermedades de las Aves de Corral / Infestaciones por Garrapatas / Borrelia / Argas Tipo de estudio: Diagnostic_studies Límite: Animals País/Región como asunto: Africa Idioma: En Revista: Comp Immunol Microbiol Infect Dis Año: 2020 Tipo del documento: Article País de afiliación: Argelia Pais de publicación: Reino Unido