Measurement of biological disulfides by postcolumn sulfitolysis following separation by HPLC.
Anal Biochem
; 172(2): 479-83, 1988 Aug 01.
Article
en En
| MEDLINE
| ID: mdl-3189788
ABSTRACT
We developed a method to measure disulfides which is applicable to biological fluids. It consisted of two parts. First, certain thiols and disulfides were separated by HPLC. Second, the eluted materials were submitted to postcolumn reaction with 2-nitro-5-thiosulfobenzoate in the presence of sulfite. The resultant yellow product, 2-nitro-5-thiobenzoate, was measured by its absorbance at 412 nm. We determined the elution characteristics of the thiols and disulfides derived from cysteine, glutathione, alpha-mercaptopropionylglycine (Thiola), and cysteamine. Penicillamine and its disulfide did not react. Cystine in the urine of 22 cystinuric patients, measured by this method, was compared with results obtained by automatic amino acid analysis.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Compuestos de Sulfhidrilo
/
Cromatografía Líquida de Alta Presión
/
Disulfuros
Límite:
Humans
Idioma:
En
Revista:
Anal Biochem
Año:
1988
Tipo del documento:
Article
País de afiliación:
Canadá