Detection, Expansion, and Isolation of Human MAIT Cells.
Methods Mol Biol
; 2111: 285-293, 2020.
Article
en En
| MEDLINE
| ID: mdl-31933215
ABSTRACT
Mucosal-associated invariant T (MAIT) cells are a novel subset of innate-like T cells that recognize vitamin B metabolites from a range of microbes presented by MHC class I-related molecules (MR1). The term mucosal-associated invariant T cells derives from the fact that MAIT cells are abundant in the liver and mucosal tissues, and human MAIT cells use a semi-invariant TCR Vα7.2 Jα33 paired with Vß2 or Vß13. Here, based on the interaction between MAIT cell and its ligand 5-OP-RU/MR1, we describe the protocols for identification, rapid expansion, and isolation of human MAIT cells.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Antígenos de Histocompatibilidad Clase I
/
Antígenos de Histocompatibilidad Menor
/
Técnicas de Cultivo de Célula
/
Células T Invariantes Asociadas a Mucosa
Tipo de estudio:
Diagnostic_studies
Límite:
Humans
Idioma:
En
Revista:
Methods Mol Biol
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2020
Tipo del documento:
Article
País de afiliación:
China