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Quantitative analysis of Anaplasma marginale acquisition and transmission by Dermacentor andersoni fed in vitro.
Vimonish, Rubikah; Johnson, Wendell C; Mousel, Michelle R; Brayton, Kelly A; Scoles, Glen A; Noh, Susan M; Ueti, Massaro W.
Afiliación
  • Vimonish R; Program in Vector-borne Diseases, Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington, 99164, USA.
  • Johnson WC; Animal Diseases Research Unit, USDA-ARS, Pullman, Washington, 99164, USA.
  • Mousel MR; Animal Diseases Research Unit, USDA-ARS, Pullman, Washington, 99164, USA.
  • Brayton KA; The Paul G. Allen School for Global Animal Health, Washington State University, Pullman, Washington, 99164, USA.
  • Scoles GA; Program in Vector-borne Diseases, Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington, 99164, USA.
  • Noh SM; Animal Diseases Research Unit, USDA-ARS, Pullman, Washington, 99164, USA.
  • Ueti MW; USDA, ARS, Invasive Insect Biocontrol and Behavior Lab, Beltsville, MD, 20705, USA.
Sci Rep ; 10(1): 470, 2020 01 16.
Article en En | MEDLINE | ID: mdl-31949241
In this study, we describe a new in vitro tick feeding system that facilitates the study of ticks and tick-borne pathogens. To optimize the system, we used Dermacentor andersoni and Anaplasma marginale as a tick-pathogen interaction model. Ticks were fed on bovine blood containing 10-fold dilutions of the pathogen to determine the effect of dose on tick infection rate. After feeding on infected blood, ticks were transferred to uninfected blood to stimulate bacterial replication within the tick vector. During stimulation feeding, blood samples were collected daily to determine if infected ticks secreted viable A. marginale. The results demonstrated similar attachment rates between the first and second tick feeding. Tick midgut and salivary glands were infected with A. marginale. However, salivary gland infection rates decreased as the percentage of parasitized erythrocytes decreased during tick acquisition feeding. Bacteria recovered from the in vitro system were able to infect a naïve bovine host. Using the highly transmissible A. marginale St. Maries strain, we demonstrated that the artificial tick feeding system is a suitable tool to study tick-pathogen interactions and that A. marginale tick salivary gland infection is dose dependent. This work demonstrates the utility of an artificial tick feeding system to directly study the association between the number of acquired pathogens and transmissibility by ticks.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Vectores Arácnidos / Infestaciones por Garrapatas / Enfermedades de los Bovinos / Anaplasma marginale / Dermacentor / Conducta Alimentaria / Infecciones por Anaplasmataceae Límite: Animals Idioma: En Revista: Sci Rep Año: 2020 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Vectores Arácnidos / Infestaciones por Garrapatas / Enfermedades de los Bovinos / Anaplasma marginale / Dermacentor / Conducta Alimentaria / Infecciones por Anaplasmataceae Límite: Animals Idioma: En Revista: Sci Rep Año: 2020 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido