Effect of the alteration of Tribbles homologue 3 expression on epithelialmesenchymal transition of transforming growth factor ß1induced mouse alveolar epithelial cells through the Wnt/ßcatenin signaling pathway.
Mol Med Rep
; 21(2): 615-622, 2020 02.
Article
en En
| MEDLINE
| ID: mdl-31974597
ABSTRACT
The aims of the present study were to elucidate the regulatory effect of exogenous Tribbles homologue 3 (TRB3) expression on the Wnt/ßcatenin signaling pathway and epithelialmesenchymal transition (EMT) in transforming growth factorß1 (TGFß1)induced mouse alveolar epithelial cells (MLE12) and investigate the underlying regulatory mechanisms. TRB3 expression was upregulated and downregulated using gene overexpression and RNA interference techniques, respectively. TGFß1stimulated MLE12 cells were examined for EMT and activation condition of the Wnt/ßcatenin signaling pathway using Cell Counting Kit8, flow cytometry, western blotting, reverse transcriptionquantitative PCR, ELISA and immunofluorescence techniques. During TGFß1induced EMT, TRB3 expression was found to be significantly upregulated (P<0.05). In the TRB3 overexpression group, upregulated expression of ßcatenin and EMTrelated genes and proteins was observed (P<0.05), and an increase in fibrosisrelated factors in the cell culture supernatant was detected (P<0.05); however, the results were the opposite in the TRB3 downregulated group (P<0.05). TRB3 may be involved in the regulation of EMT in TGFß1induced MLE12 cells through the Wnt/ßcatenin signaling pathway.
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Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Proteínas de Ciclo Celular
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Factor de Crecimiento Transformador beta1
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Células Epiteliales Alveolares
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Transición Epitelial-Mesenquimal
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Vía de Señalización Wnt
Límite:
Animals
Idioma:
En
Revista:
Mol Med Rep
Año:
2020
Tipo del documento:
Article