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Comparative analysis of Lactobacillus gasseri from Chinese subjects reveals a new species-level taxa.
Zhou, Xingya; Yang, Bo; Stanton, Catherine; Ross, R Paul; Zhao, Jianxin; Zhang, Hao; Chen, Wei.
Afiliación
  • Zhou X; State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, China.
  • Yang B; School of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi, 214122, China.
  • Stanton C; State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, China. bo.yang@jiangnan.edu.cn.
  • Ross RP; School of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi, 214122, China. bo.yang@jiangnan.edu.cn.
  • Zhao J; International Joint Research Center for Probiotics & Gut Health, Jiangnan University, Wuxi, Jiangsu, China. bo.yang@jiangnan.edu.cn.
  • Zhang H; International Joint Research Center for Probiotics & Gut Health, Jiangnan University, Wuxi, Jiangsu, China.
  • Chen W; Teagasc Food Research Centre, Moorepark, Fermoy, Cork, Ireland.
BMC Genomics ; 21(1): 119, 2020 Feb 03.
Article en En | MEDLINE | ID: mdl-32013858
BACKGROUND: Lactobacillus gasseri as a probiotic has history of safe consumption is prevalent in infants and adults gut microbiota to maintain gut homeostasis. RESULTS: In this study, to explore the genomic diversity and mine potential probiotic characteristics of L. gasseri, 92 strains of L. gasseri were isolated from Chinese human feces and identified based on 16 s rDNA sequencing, after draft genomes sequencing, further average nucleotide identity (ANI) value and phylogenetic analysis reclassified them as L. paragasseri (n = 79) and L. gasseri (n = 13), respectively. Their pan/core-genomes were determined, revealing that L. paragasseri had an open pan-genome. Comparative analysis was carried out to identify genetic features, and the results indicated that 39 strains of L. paragasseri harboured Type II-A CRISPR-Cas system while 12 strains of L. gasseri contained Type I-E and II-A CRISPR-Cas systems. Bacteriocin operons and the number of carbohydrate-active enzymes were significantly different between the two species. CONCLUSIONS: This is the first time to study pan/core-genome of L. gasseri and L. paragasseri, and compare their genetic diversity, and all the results provided better understating on genetics of the two species.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Lactobacillus gasseri Tipo de estudio: Incidence_studies / Prognostic_studies Límite: Adult / Humans / Infant Idioma: En Revista: BMC Genomics Asunto de la revista: GENETICA Año: 2020 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Lactobacillus gasseri Tipo de estudio: Incidence_studies / Prognostic_studies Límite: Adult / Humans / Infant Idioma: En Revista: BMC Genomics Asunto de la revista: GENETICA Año: 2020 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido