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A simple and reliable method for determination of optimum pH in coupled enzyme assays.
Bowman, Lee; Motamed, Rachael; Lee, Paul; Aleem, Kadijah; Berawala, Astha S; Hayden, Katherine L; Bzik, David J; Chattopadhyay, Debasish.
Afiliación
  • Bowman L; Department of Chemistry & Physics, Birmingham-Southern College, Birmingham, AL 35254, USA.
  • Motamed R; Department of Chemistry & Physics, Birmingham-Southern College, Birmingham, AL 35254, USA.
  • Lee P; Department of Medicine, University of Alabama at Birmingham, Birmingham, AL 35294, USA.
  • Aleem K; Department of Medicine, University of Alabama at Birmingham, Birmingham, AL 35294, USA.
  • Berawala AS; Department of Chemistry & Physics, Birmingham-Southern College, Birmingham, AL 35254, USA.
  • Hayden KL; Department of Chemistry & Physics, Birmingham-Southern College, Birmingham, AL 35254, USA.
  • Bzik DJ; Department of Microbiology & Immunology, Dartmouth Geisel School of Medicine, Lebanon, NH 03756, USA.
  • Chattopadhyay D; Department of Medicine, University of Alabama at Birmingham, Birmingham, AL 35294, USA.
Biotechniques ; 68(4): 200-203, 2020 04.
Article en En | MEDLINE | ID: mdl-32056453
ABSTRACT
Determination of the optimum pH in a coupled enzyme assay poses significant challenges because altering the pH of the reaction mixture can affect the performance of both enzymes. Here, we demonstrate a simple and reliable method to determine the pH optimum for pyruvate kinase using the pyruvate kinase/lactate dehydrogenase coupled enzyme assay. This simple and reliable method can be broadly adapted to determine the pH optimum for various enzymes that are assayed using a coupled enzyme assay.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Pruebas de Enzimas / Concentración de Iones de Hidrógeno Idioma: En Revista: Biotechniques Año: 2020 Tipo del documento: Article País de afiliación: Estados Unidos
Texto completo
Añadir a Mi BVS
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XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Pruebas de Enzimas / Concentración de Iones de Hidrógeno Idioma: En Revista: Biotechniques Año: 2020 Tipo del documento: Article País de afiliación: Estados Unidos