TF protein of Sindbis virus antagonizes host type I interferon responses in a palmitoylation-dependent manner.
Virology
; 542: 63-70, 2020 03.
Article
en En
| MEDLINE
| ID: mdl-32056669
ABSTRACT
Sindbis virus (SINV) produces the small membrane protein TF from the 6K gene via a (-1) programmed ribosomal frameshifting. While several groups have shown that TF-deficient virus exhibits reduced virulence, the mechanism(s) by which this occurs remain unknown. Here, we demonstrate a role for TF in antagonizing the host interferon response. Using wild-type and type 1 interferon receptor-deficient mice and primary cells derived from these animals, we show that TF controls the induction of the host interferon response at early times during infection. Loss of TF production leads to elevated interferon and a concurrent reduction in viral loads with a loss of pathogenicity. Palmitoylation of TF has been shown to be important for particle assembly and morphology. We find that palmitoylation of TF also contributes to the ability of TF to antagonize host interferon responses as dysregulated palmitoylation of TF reduces virulence in a manner similar to loss of TF.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Virus Sindbis
/
Proteínas Virales
/
Interferón Tipo I
Límite:
Animals
Idioma:
En
Revista:
Virology
Año:
2020
Tipo del documento:
Article
País de afiliación:
Estados Unidos