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CCOAOMT1, a candidate cargo secreted via VAMP721/722 secretory vesicles in Arabidopsis.
Kwon, Hyeokjin; Cho, Da Jeong; Lee, Horim; Nam, Myung Hee; Kwon, Chian; Yun, Hye Sup.
Afiliación
  • Kwon H; Department of Biological Sciences, Konkuk University, Seoul, 05029, South Korea.
  • Cho DJ; Department of Molecular Biology, Dankook University, Cheonan, 31116, South Korea.
  • Lee H; Department of Biotechnology, Duksung Women's University, Seoul, 01369, South Korea.
  • Nam MH; Environmental Risk and Welfare Research Team, Korea Basic Science Institute (KBSI), Seoul, 02855, South Korea.
  • Kwon C; Department of Molecular Biology, Dankook University, Cheonan, 31116, South Korea.
  • Yun HS; Department of Biological Sciences, Konkuk University, Seoul, 05029, South Korea. Electronic address: hsyun@konkuk.ac.kr.
Biochem Biophys Res Commun ; 524(4): 977-982, 2020 04 16.
Article en En | MEDLINE | ID: mdl-32059845
ABSTRACT
We previously found that VAMP721/722 SNARE proteins guide secretory vesicles to pathogen-attacking sites during immune responses in Arabidopsis, which suggests that these vesicles should deliver immune molecules. However, the lethality of vamp721 vamp722 double null mutant makes it difficult to understand the nature of cargo transported via VAMP721/722 vesicles. Since VAMP721/722-depleted (VAMP721+/-VAMP722-/- and VAMP721-/-VAMP722+/-) plants show compromised resistance to extracellular pathogens, we assume that an immune protein secreted through the VAMP721/722-engaged exocytosis would be remained more in VAMP721/722-depleted plants than WT. By comparing intracellular proteins between WT and VAMP721/722-depleted plants, we found caffeoyl-CoA O-methyltransferase 1 (CCOAOMT1) involved in the lignin biosynthesis was more abundantly detected in both VAMP721/722-depleted lines than WT. Plants are well-known to deposit secondary cell walls as physical barriers at pathogen-attempting sites. Therefore, extracellular detection of CCOAOMT1 and impaired resistance to Pseudomonas syringae DC3000 in ccoaomt1 plants suggest that plants secrete cell wall-modifying enzymes at least including CCOAOMT1 to reinforce the secondary cell walls for immunity.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Arabidopsis / Proteínas de Arabidopsis / Proteínas R-SNARE / Metiltransferasas Idioma: En Revista: Biochem Biophys Res Commun Año: 2020 Tipo del documento: Article País de afiliación: Corea del Sur

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Arabidopsis / Proteínas de Arabidopsis / Proteínas R-SNARE / Metiltransferasas Idioma: En Revista: Biochem Biophys Res Commun Año: 2020 Tipo del documento: Article País de afiliación: Corea del Sur