An electronic enzyme-linked immunosorbent assay platform for protein analysis based on magnetic beads and AlGaN/GaN high electron mobility transistors.
Analyst
; 145(7): 2725-2730, 2020 Apr 07.
Article
en En
| MEDLINE
| ID: mdl-32077869
ABSTRACT
AlGaN/GaN high electron mobility transistor (HEMT) biosensors have attracted attention due to their high sensitivity, stability, and fast response characteristics. Some related studies have been explored but a Debye screening problem exists in physiological solutions hindering the detection of bio-macromolecules. Herein, a novel fast analytical platform for electronic enzyme-linked immunosorbent assay (e-ELISA) is proposed based on AlGaN/GaN HEMT with magnetic beads (MBs); MB-based e-ELISA decouples the modified area from the sensing surface to simplify the assay. Combining the advantages of e-ELISA and MBs, the resulting analytical platform presents a sensing capability beyond the Debye-screening limit and a novel ability to be reused. This platform offers a fast response toward prostate specific antigen (PSA) and the lowest concentration of detection is 1 fg mL-1. Compared with conventional AlGaN/GaN HEMT biosensors, it shows higher sensitivity (3.73 µA dec-1) in a linear range (1 fg mL-1 to 1 pg mL-1), which is within the constraints of emergency care applications. The platform's high sensitivity and fast repeatability endow it with great potential for early and rapid diagnosis.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Transistores Electrónicos
/
Ensayo de Inmunoadsorción Enzimática
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Técnicas Biosensibles
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Antígeno Prostático Específico
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Compuestos de Aluminio
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Galio
Límite:
Humans
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Male
Idioma:
En
Revista:
Analyst
Año:
2020
Tipo del documento:
Article